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NPAS2 Antibody (PA5-95462) in WB
Western blot analysis of NPAS2 in Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate, Lane 3: human COLO-320 whole cell lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with NPAS2 polyclonal antibody (Product # PA5-95462) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and develo... View More
产品信息
PA5-95462
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human NPAS2 recombinant protein (Position: D474-Q578).
偶联物
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2807265
产品详细信息
Human NPAS2 shares 88.1% amino acid (aa) sequence identity with mouse NPAS2.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
Members of the basic helix-loop-helix-PER-ARNT-SIM (bHLH-PAS) family are transcription factors that contain a bHLH DNA binding domain located amino-terminal to a PAS domain. Neuronal PAS domain protein 2 (NPAS2, also designated PAS 4/MOP4) is a member of the bHLH-PAS family and the PAS superfamily. NPAS2, which maps to chromosome 2p11.2-2q13, is expressed primarily in the neurons during the first week of postnatal development. The pattern of NPAS2 expression temporally matches the development of learning and memory, and spatially matches the frontal association/limbic forebrain pathway. NPAS2 may serve a regulatory role in the development and maintenance of long-term memory, and may be required for the processing of complex sensory information. NPAS2 and MOP3 form a transcriptionally active heterodimer which binds to a CACGTGA-containing DNA element and drives transcription from a linked luciferase reporter gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Basic-helix-loop-helix-PAS protein MOP4; bHLHe9; Class E basic helix-loop-helix protein 9; Member of PAS protein 4; member of PAS superfamily 4; Neuronal PAS domain-containing protein 2; Neuronal PAS2; PAS domain-containing protein 4
基因别名: BHLHE9; MOP4; NPAS2; PASD4
UniProt ID: (Human) Q99743, (Mouse) P97460
Entrez Gene ID: (Human) 4862, (Mouse) 18143, (Rat) 316351
transcription, DNA-templated
cellular response to DNA damage stimulus
central nervous system development
circadian rhythm
circadian regulation of gene expression
positive regulation of DNA repair
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to redox state
negative regulation of cell death
regulation of response to DNA damage stimulus
regulation of transcription, DNA-templated
signal transduction
circadian sleep/wake cycle
regulation of circadian rhythm
locomotor rhythm
rhythmic process
