Invitrogen

NPY Monoclonal Antibody (8)

NPY Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

NPY Antibody (ABS 028-08-02) in ICC/IF

Immunofluorescence analysis of NPY was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NPY Monoclonal Antibody (8) (Product # ABS 028-08-02) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), ... View More

NPY Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NPY Antibody in Immunohistochemistry (IHC)

NPY Antibody in Immunohistochemistry - Free Floating (IHC (Free))

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产品信息

ABS 028-08-02

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫组织化学（悬浮细胞） (IHC (Free))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Pig

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

抗原

Synthetic peptide made to human/rat NPY.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4°C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_1077304

产品详细信息

ABS 028-08-02 detects Neuropeptide Y in human and rat samples.

ABS 028-08-02 has been successfully used in ELISA and immunohistochemistry (paraffin) procedures.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

靶标信息

This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase, regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Endometritis Changes the Neurochemical Characteristics of the Caudal Mesenteric Ganglion Neurons Supplying the Gilt Uterus.

Animals : an open access journal from MDPI

Jana B,Całka J

Published figure using NPY monoclonal antibody (Product # ABS 028-08-02) in Immunohistochemistry

Wed May 20 00:00:00 UTC 2020

Endometritis Changes the Neurochemical Characteristics of the Caudal Mesenteric Ganglion Neurons Supplying the Gilt Uterus.

Animals : an open access journal from MDPI

Jana B,Całka J

Published figure using NPY monoclonal antibody (Product # ABS 028-08-02) in Immunohistochemistry

Wed May 20 00:00:00 UTC 2020

Ghrelin Regulates Glucose and Glutamate Transporters in Hypothalamic Astrocytes.

Scientific reports

Fuente-Martín E,García-Cáceres C,Argente-Arizón P,Díaz F,Granado M,Freire-Regatillo A,Castro-González D,Ceballos ML,Frago LM,Dickson SL,Argente J,Chowen JA

ABS 028-08-02 was used in immunohistochemistry - free floating to study how glucose and glutamate transporters are regulated by Ghrelin in hypothalamic astrocytes