Invitrogen

NRGN Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他8个NRGN抗体

说明书

实验方案

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说明书

实验方案

技术支持

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NRGN Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of NRGN was performed using PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NRGN Rabbit Polyclonal Antibody (Product # PA5-76392) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing Cytoplasmic localization of NRGN. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

NRGN Antibody in Western Blot (WB) — Western blot was performed using Anti-NRGN Polyclonal Antibody (Product # PA5-76392) and a 13 kDa band corresponding to NRGN was observed across the cell lines and tissues tested except Mouse Skin, Mouse and Rat Lung. Whole cell extracts (30 µg lysate) of PC-3 (Lane 1), HEL 92.1.7 (Lane 2) and tissue extracts of Mouse Brain (Lane 3), Rat Brain (Lane 4), Mouse Skin (Lane 5), Mouse Lung (Lane 6) and Rat Lung (Lane 7) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence using Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

NRGN Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. PC-3 cells were transfected with NRGN siRNA and loss of signal was observed in Western Blot using NRGN Polyclonal Antibody (Product # PA5-76392). {KD}

产品信息

PA5-76392

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫细胞化学 (ICC/IF)	5 µg/mL	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant protein of human NRGN.
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.2, with 50% glycerol
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2720119

产品详细信息

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

靶标信息

Neurogranin is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: calmodulin-binding protein; NEUG(55-78); Neurogranin; neurogranin (protein kinase C substrate, RC3); Ng; postsynaptic spine 1; protein kinase C substrate; Protein kinase C substrate 7.5 kDa protein; RC3

基因别名: 0710001B06Rik; AI838505; hng; NG; NG/RC3; NRGN; Pss1; R75334; RC3

UniProt ID: (Human) Q92686, (Mouse) P60761, (Rat) Q04940

Entrez Gene ID: (Human) 4900, (Mouse) 64011, (Rat) 64356

功能

nervous system development intracellular signal transduction positive regulation of long-term synaptic potentiation signal transduction associative learning telencephalon development

参与通路

calmodulin binding phosphatidylinositol-3,4,5-trisphosphate binding phosphatidic acid binding

NRGN Polyclonal Antibody

产品信息

PA5-76392

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

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保存液

内含物

保存条件

RRID

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