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GenScript
NWSHPQFEK Tag Monoclonal Antibody (5A9F9), THE™
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NWSHPQFEK Tag Antibody (A01732-100) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Strep II tagged protein transfected HEK293 cells using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (Cat. No. A01732) and Mouse IgG Control (Whole Molecule), Purified (Cat. No. A01007). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
产品信息
A01732-100
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
5A9F9
抗原
NWSHPQFEK peptide conjugated to KLH
偶联物
形式
Lyophilized
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag II fusion proteins.
靶标信息
The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in various fashions. The original Strep-tag was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
