Invitrogen

Nanog Monoclonal Antibody (eBioMLC-51), eFluor™ 660, eBioscience™

Nanog Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 76

Nanog Antibody (50-5761-82) in ICC/IF

Immunocytochemistry of fixed and permeabilized F9 cells stained with 10 µg/mL Rat IgG2a K Isotype Control eFluor® 660 (left) or 10 µg/mL Anti-Mouse Nanog eFluor® 660 (right). Nuclei are stained with DAPI; colocalization of signal appears pink.

Nanog Antibody in Immunohistochemistry (IHC)

Nanog Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

Nanog Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Nanog Antibody in Western Blot, Immunocytochemistry, Flow Cytometry (WB, ICC/IF, Flow)

Nanog Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

产品信息

50-5761-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

查看8篇已发表文章 8篇已发表文章

免疫组化 (IHC)

Assay-Dependent

查看7篇已发表文章 7篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

10 µg/mL

查看29篇已发表文章 29篇已发表文章

流式细胞分析 (Flow)

0.06 µg/test

查看8篇已发表文章 8篇已发表文章

染色质免疫沉淀实验 (ChIP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse, Rat

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioMLC-51

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574243

产品详细信息

Description: The eBioMLC51 monoclonal antibody recognizes mouse Nanog. Nanog is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3. Expression of Nanog in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. Nanog expression often overlaps, but is not identical to, that of Oct4. Nanog is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells.

Nanog acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus. The CD2 domain is unique to Nanog, whereas the NK2 DNA binding domain is well conserved. Immunoblotting using eBioMLC51 reveals a band at ~ 45 kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen. Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Product # 00-5523, for intracellular staining of mouse Nanog.

Applications Reported: This eBioMLC-51 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, microscopy, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This eBioMLC-51 antibody has been tested by immunocytochemistry on fixed and permeabilized cells and can be used at less than or equal to 10 µg/mL. The eBioMLC-51 antibody has also been tested by intracellular staining followed by flow cytometric analysis of the F9 cell line using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.

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Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.

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Tsakiridis A,Huang Y,Blin G,Skylaki S,Wymeersch F,Osorno R,Economou C,Karagianni E,Zhao S,Lowell S,Wilson V

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Development (Cambridge, England)

Le Bin GC,Muñoz-Descalzo S,Kurowski A,Leitch H,Lou X,Mansfield W,Etienne-Dumeau C,Grabole N,Mulas C,Niwa H,Hadjantonakis AK,Nichols J

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Formation of a polarised primitive endoderm layer in embryoid bodies requires fgfr/erk signalling.

PloS one

Doughton G,Wei J,Tapon N,Welham MJ,Chalmers AD

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AID stabilizes stem-cell phenotype by removing epigenetic memory of pluripotency genes.

Nature

Kumar R,DiMenna L,Schrode N,Liu TC,Franck P,Muñoz-Descalzo S,Hadjantonakis AK,Zarrin AA,Chaudhuri J,Elemento O,Evans T

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Thu Aug 01 00:00:00 UTC 2013

A defined Oct4 level governs cell state transitions of pluripotency entry and differentiation into all embryonic lineages.

Nature cell biology

Radzisheuskaya A,Chia Gle B,dos Santos RL,Theunissen TW,Castro LF,Nichols J,Silva JC

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Immunofluorescence

Sat Jun 01 00:00:00 UTC 2013

A defined Oct4 level governs cell state transitions of pluripotency entry and differentiation into all embryonic lineages.

Nature cell biology

Radzisheuskaya A,Chia Gle B,dos Santos RL,Theunissen TW,Castro LF,Nichols J,Silva JC

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Immunofluorescence

Sat Jun 01 00:00:00 UTC 2013

Long-range chromatin contacts in embryonic stem cells reveal a role for pluripotency factors and polycomb proteins in genome organization.

Cell stem cell

Denholtz M,Bonora G,Chronis C,Splinter E,de Laat W,Ernst J,Pellegrini M,Plath K

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Immunocytochemistry

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Totipotent embryonic stem cells arise in ground-state culture conditions.

Cell reports

Morgani SM,Canham MA,Nichols J,Sharov AA,Migueles RP,Ko MS,Brickman JM

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Flow Cytometry

Thu Jun 27 00:00:00 UTC 2013

Totipotent embryonic stem cells arise in ground-state culture conditions.

Cell reports

Morgani SM,Canham MA,Nichols J,Sharov AA,Migueles RP,Ko MS,Brickman JM

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Flow Cytometry

Thu Jun 27 00:00:00 UTC 2013

The transcriptional and epigenomic foundations of ground state pluripotency.

Cell

Marks H,Kalkan T,Menafra R,Denissov S,Jones K,Hofemeister H,Nichols J,Kranz A,Stewart AF,Smith A,Stunnenberg HG

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Flow Cytometry

Fri Apr 27 00:00:00 UTC 2012

The transcriptional and epigenomic foundations of ground state pluripotency.

Cell

Marks H,Kalkan T,Menafra R,Denissov S,Jones K,Hofemeister H,Nichols J,Kranz A,Stewart AF,Smith A,Stunnenberg HG

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Flow Cytometry

Fri Apr 27 00:00:00 UTC 2012

Correlations between the levels of Oct4 and Nanog as a signature for naïve pluripotency in mouse embryonic stem cells.

Stem cells (Dayton, Ohio)

Muñoz Descalzo S,Rué P,Garcia-Ojalvo J,Martinez Arias A

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Immunofluorescence

Sat Dec 01 00:00:00 UTC 2012

Histone variant macroH2A marks embryonic differentiation in vivo and acts as an epigenetic barrier to induced pluripotency.

Journal of cell science

Pasque V,Radzisheuskaya A,Gillich A,Halley-Stott RP,Panamarova M,Zernicka-Goetz M,Surani MA,Silva JC

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Immunofluorescence

Sat Dec 15 00:00:00 UTC 2012

Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation.

Nature cell biology

Wray J,Kalkan T,Gomez-Lopez S,Eckardt D,Cook A,Kemler R,Smith A

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Immunofluorescence

Sun Jun 19 00:00:00 UTC 2011

A PiggyBac-based recessive screening method to identify pluripotency regulators.

PloS one

Guo G,Huang Y,Humphreys P,Wang X,Smith A

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Flow Cytometry

Mon Apr 18 00:00:00 UTC 2011

A PiggyBac-based recessive screening method to identify pluripotency regulators.

PloS one

Guo G,Huang Y,Humphreys P,Wang X,Smith A

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Flow Cytometry

Mon Apr 18 00:00:00 UTC 2011

A PiggyBac-based recessive screening method to identify pluripotency regulators.

PloS one

Guo G,Huang Y,Humphreys P,Wang X,Smith A

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Flow Cytometry

Mon Apr 18 00:00:00 UTC 2011

Dioxin exposure disrupts the differentiation of mouse embryonic stem cells into cardiomyocytes.

Toxicological sciences : an official journal of the Society of Toxicology

Wang Y,Fan Y,Puga A

Published figure using Nanog monoclonal antibody (Product # 50-5761-82) in Western Blot

Sat May 01 00:00:00 UTC 2010

生物信息学

蛋白别名: early embryo specific expression NK family; Early embryo specific expression NK-type homeobox protein; ES cell-associated protein 4; FLJ12581; HGNC:20857; Homeobox protein NANOG; Homeobox transcription factor Nanog

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基因别名: 2410002E02Rik; Ecat4; ENK; Nanog

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UniProt ID: (Mouse) Q80Z64

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Entrez Gene ID: (Mouse) 71950

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功能

enhancer sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding sequence-specific DNA binding transcription regulatory region DNA binding homeodomain transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter mesodermal cell fate commitment endodermal cell fate specification transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development cell proliferation positive regulation of cell proliferation embryo development response to organic substance negative regulation of cell fate commitment regulation of gene expression stem cell division stem cell population maintenance negative regulation of BMP signaling pathway response to retinoic acid somatic stem cell population maintenance negative regulation of endodermal cell fate specification regulation of cell differentiation negative regulation of cell differentiation positive regulation of transcription, DNA-templated positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter stem cell differentiation

Nanog Monoclonal Antibody (eBioMLC-51), eFluor™ 660, eBioscience™

Nanog Antibody (50-5761-82) in ICC/IF

产品信息

50-5761-82

免疫印迹 (WB)

免疫组化 (IHC)

免疫组织化学（多聚甲醛固定） (IHC (PFA))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

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生物信息学

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Nanog Monoclonal Antibody (eBioMLC-51), eFluor™ 660, eBioscience™

Nanog Antibody (50-5761-82) in ICC/IF

产品信息

50-5761-82

免疫印迹 (WB)

免疫组化 (IHC)

免疫组织化学（多聚甲醛固定） (IHC (PFA))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

染色质免疫沉淀 实验 (ChIP)

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宿主/亚型

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偶联物

激发/发射光谱

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纯化类型

保存液

内含物

保存条件

运输条件

RRID

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染色质免疫沉淀实验 (ChIP)