Invitrogen

Nectin 3 Polyclonal Antibody

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说明书
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说明书
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问题与答案
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说明书
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Nectin 3 Polyclonal Antibody

产品信息

PA5-144003

应用
建议稀释比
已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL
-

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL
-

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells
-

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL
-
产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Nectin 3/NECTIN3 recombinant protein (Position: E92-N296).
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_3075217

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human U20S whole cellhuman HEK293 whole cell. IHC: human rectal cancer tissue. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calciumindependent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3 alpha, -3 beta and -3 gamma, of which nectin-3 alpha is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD113; CDw113; DKFZP566B0846; FLJ90624; nectin 3; Nectin cell adhesion molecule 3; Nectin-3; poliovirus receptor-related 3; Poliovirus receptor-related protein 3

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基因别名: CD113; CDW113; NECTIN-3; NECTIN3; PPR3; PRR3; PVRL3; PVRR3

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UniProt ID: (Human) Q9NQS3

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Entrez Gene ID: (Human) 25945

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