Invitrogen

NeuroD1 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

查看其他12个NeuroD1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (6)
高级验证 (1)

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NeuroD1 Antibody (PA5-78075) in WB

Western blot was performed using Anti-NeuroD1 Polyclonal Antibody (Product # PA5-78075) and a 45 kDa band corresponding to NEUROD1 was observed only in the IMR-32 cell line, that expresses NeuroD1 at high levels. Nuclear enriched extracts (30 µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), U-87 MG (Lane 3), SK-BR-3 (Lane 4), Caco-2 (Lane 5), HT-29 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot®... View More

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NeuroD1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of NeuroD1 was performed using 70% confluent log phase IMR-32. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NeuroD1 Rabbit Polyclonal Antibody (Product # PA5-78075) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

NeuroD1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry (Frozen) analysis of NeuroD1 was performed in frozen-sectioned rat E13.5 brain tissue using NeuroD1 Polyclonal Antibody (Product # PA5-78075) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

NeuroD1 Antibody in ChIP Assay (ChIP) — Chromatin Immunoprecipitation (ChIP) assay of endogenous NeuroD1 protein using Anti-NeuroD1 Antibody: ChIP was performed using Anti-NeuroD1 Rabbit Polyclonal Antibody (Product # PA5-78075, 5 µg) on sheared chromatin from IMR32 cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to NEUROD1, JUNB, cMYC and SLIT2 transcription start sites and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

NeuroD1 Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of NeuroD1 was observed between the MYCN amplified cell line, IMR32 as opposed to SH-SY5Y and U-87 MG, which are non-MYCN amplified cell lines, using Anti-NeuroD1 Polyclonal Antibody (Product # PA5-78075) in Western Blot.(doi: 10.3727/096504021X16401852341873). The other cell lines are also of non-neuronal lineage and express NeuroD1 at a relatively lower level than IMR-32. {RE}

产品信息

PA5-78075

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

5 µg/mL

染色质免疫沉淀实验 (ChIP)

2.5 µg/10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse NeuroD1. The exact sequence is proprietary.

偶联物

Unconjugated

形式

Liquid

浓度

0.47 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2736208

产品详细信息

Positive Control: IMR32, IMR32 nuclear extract

Predicted Reactivity: Mouse (100%), Bovine (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

NeuroD1 is a transcriptional activator that acts as a differentiation factor during neurogenesis. It has been demonstrated to bind to the insulin gene E-box. Efficient DNA binding requires dimerization with another basic helix-loop-helix (bHLH) protein. Defects in NEUROD1 are a cause of maturity onset diabetes of the young type VI (MODY6). MODY6 is a form of non-insulin-dependent diabetes mellitus characterized by an autosomal dominant mode of inheritance, onset during young adulthood and a primary defect in insulin secretion.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Basic helix-loop-helix factor 1; basic helix-loop-helix transcription factor; beta-cell E-box transactivator 2; Beta-cell E-box transcriptional activator 2; Beta2; BHF-1; BHF1; bHLHa3; Class A basic helix-loop-helix protein 3; neuro d1; NeuroD; NeuroD1; neurogenic differentiation 1; Neurogenic differentiation factor 1; neurogenic helix-loop-helix protein NEUROD

基因别名: BETA2; BHF-1; BHLHA3; MODY6; NEUROD; NEUROD1

UniProt ID: (Human) Q13562, (Rat) Q64289, (Mouse) Q60867

Entrez Gene ID: (Human) 4760, (Rat) 29458, (Mouse) 18012

分子生物学功能: basic helix-loop-helix transcription factor

功能

pancreatic A cell fate commitment pancreatic PP cell fate commitment transcription from RNA polymerase II promoter nucleocytoplasmic transport nitric oxide mediated signal transduction response to glucose anterior/posterior pattern specification dentate gyrus development cerebellum development neurogenesis signal transduction involved in regulation of gene expression insulin secretion endocrine pancreas development amacrine cell differentiation enteroendocrine cell differentiation response to drug glucose homeostasis positive regulation of apoptotic process positive regulation of cell differentiation positive regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of JAK-STAT cascade embryonic organ morphogenesis neuron development inner ear development regulation of insulin secretion positive regulation of sequence-specific DNA binding transcription factor activity regulation of intestinal epithelial structure maintenance regulation of cell cycle arrest cellular response to glucose stimulus negative regulation of type B pancreatic cell apoptotic process positive regulation of transcription regulatory region DNA binding transcription, DNA-templated hindbrain development camera-type eye development cell fate commitment regulation of neuron differentiation regulation of transcription, DNA-templated multicellular organism development nervous system development cell differentiation

参与通路

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding sequence-specific DNA binding protein heterodimerization activity E-box binding DNA binding double-stranded DNA binding protein dimerization activity

NeuroD1 Polyclonal Antibody

NeuroD1 Antibody (PA5-78075) in WB

产品信息

PA5-78075

免疫印迹 (WB)

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

染色质免疫沉淀实验 (ChIP)

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

篇参考文献 (0)

生物信息学

性能保障

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NeuroD1 Polyclonal Antibody

NeuroD1 Antibody (PA5-78075) in WB

产品信息

PA5-78075

免疫印迹 (WB)

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

染色质免疫沉淀 实验 (ChIP)

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

篇参考文献 (0)

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)