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Noggin Antibody (PA5-95474) in WB
Western blot analysis of Noggin in Lane 1: human HeLa whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A375 whole cell lysates, Lane 5: human 22RV1 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with Noggin polyclonal antibody (Product # PA5-95474) using a 0.5 µg... View More
Product Details
PA5-95474
Applications
Tested Dilution
Publications
产品规格
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
E.coli-derived human Noggin recombinant protein (Position: L43-T153).
Conjugate
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2807276
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. The morphogenesis of organs is initiated by a downgrowth from a layer of epithelial stem cells. This process is achieved through the receipt of signals from 1) a WNT protein (WNT3A) to stabilize beta-catenin; and 2) Noggin, which is a bone morphogenetic protein inhibitor. Noggin mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have been identified, which have multiple joint fusion as their principal defect.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: Noggin; Noggin precursor; symphalangism 1 (proximal)
Gene Aliases: NOG; SYM1; SYNS1; SYNS1A
UniProt ID: (Human) Q13253, (Mouse) P97466
Entrez Gene ID: (Human) 9241, (Rat) 25495, (Mouse) 18121
Molecular Function:
intercellular signal molecule
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
osteoblast differentiation
in utero embryonic development
endoderm formation
mesoderm formation
epithelial to mesenchymal transition
neural plate morphogenesis
neural tube closure
nervous system development
motor neuron axon guidance
dorsal/ventral pattern formation
spinal cord development
cell differentiation in hindbrain
pituitary gland development
negative regulation of cell migration
BMP signaling pathway
negative regulation of BMP signaling pathway
somatic stem cell population maintenance
wound healing
middle ear morphogenesis
embryonic digit morphogenesis
regulation of neuron differentiation
negative regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
axial mesoderm development
notochord morphogenesis
embryonic skeletal system development
negative regulation of astrocyte differentiation
positive regulation of epithelial cell proliferation
cartilage development
negative regulation of cardiac muscle cell proliferation
limb development
embryonic skeletal joint morphogenesis
negative regulation of cytokine activity
face morphogenesis
negative regulation of pathway-restricted SMAD protein phosphorylation
lung morphogenesis
prostatic bud formation
ureteric bud formation
fibroblast growth factor receptor signaling pathway involved in neural plate anterior/posterior pattern formation
negative regulation of cartilage development
somite development
negative regulation of canonical Wnt signaling pathway
positive regulation of branching involved in ureteric bud morphogenesis
positive regulation of glomerulus development
regulation of fibroblast growth factor receptor signaling pathway involved in neural plate anterior/posterior pattern formation
negative regulation of apoptotic signaling pathway
urogenital system development
ureteric bud development
pattern specification process
axon guidance
central nervous system development
brain development
negative regulation of gene expression
neural tube development
regulation of BMP signaling pathway
forebrain development
anatomical structure formation involved in morphogenesis
mesenchymal cell differentiation
cellular response to BMP stimulus
multicellular organism development
cell differentiation
negative regulation of cell differentiation
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