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Invitrogen
Nucleolin Polyclonal Antibody
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Nucleolin Antibody (PA3-16875) in ICC/IF
Immunofluorescence analysis of Nucleolin was performed using 70% confluent log phase U-2 OS cells treated with Heat Shock (44 degrees for 3 hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Nucleolin Polyclonal Antibody (Product # PA3-16875) at 1:100 dil... View More
产品信息
PA3-16875
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Plant,
Rat
已发表种属
C. elegans,
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A fusion protein containing amino acids 284-709 of human Nucleolin.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
whole serum
内含物
0.1% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Heat mediated antigen retrieval is recommended for IHC on paraffin embedded tissues.
Suggested positive control: PD31 murine pre-B cells for immunofluorescence and crude PD31 nuclear extracts for Western Blot.
靶标信息
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: FLJ45706; FLJ59041; nucl antibody; Nucleolin; nucleolin-like protein; Protein C23
基因别名: B530004O11Rik; C23; D0Nds28; D1Nds28; NCL; Nuc; Nucl
UniProt ID: (Human) P19338, (Mouse) P09405
Entrez Gene ID: (Human) 4691, (Mouse) 17975, (Rat) 25135
nucleotide binding
single-stranded DNA binding
RNA binding
calcium ion binding
protein binding
protein C-terminus binding
selenocysteine insertion sequence binding
rRNA primary transcript binding
telomeric DNA binding
histone binding
identical protein binding
laminin binding
poly(A) RNA binding
ErbB-4 class receptor binding
nucleic acid binding
DNA binding
receptor binding
sequence-specific DNA binding
angiogenesis
endocytosis
spermatogenesis
positive regulation of tumor necrosis factor production
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
cellular response to lipopolysaccharide
liver regeneration
positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter
regulation of rRNA processing
positive regulation of interleukin-6 secretion
