Invitrogen

OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6), Alexa Fluor™ Plus 647

OCT4 Antibody in Immunocytochemistry (ICC/IF)

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OCT4 Antibody (701756RP647) in ICC/IF

Immunofluorescent analysis of OCT4 was performed using OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6), Alexa Fluor™ Plus 647 (Product # 701756RP647, 1:200) on 70-80% confluent NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6), Alexa Fluor™ Plus 647. Panel a) shows representative images of cells that were stained for detecti... View More

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OCT4 Antibody — Antibody specificity was demonstrated by siRNA-mediated knockdown of the target protein. NTERA-2 cells were transfected with OCT4 siRNA and a decrease in signal intensity was observed in flow cytometry application using OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6), Alexa Fluor™ Plus 647 (Product # 701756RP647). {KD}

产品信息

701756RP647

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

0.5 µg/test

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

3H8L6

抗原

Protein corresponding to Human POU5F1 (aa 1-360)

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偶联物

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

激发/发射光谱

658/675 nm 查看光谱

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A

保存液

proprietary buffer, pH 6.8

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Applications Tested: This 3H8L6 antibody has been tested by immunocytochemistry and flow cytometric analysis of NTERA-2 cells. This may be used for immunocytochemistry at 10 µg/ml and for flow cytometry at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2

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基因别名: Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4; POU5F1

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UniProt ID: (Human) Q01860

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Entrez Gene ID: (Human) 5460

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功能