Invitrogen

ORC1 Polyclonal Antibody

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ORC1 Antibody in Western Blot (WB) — Western blot analysis of ORC1 in Daudi cell lysate in the 1) absence and 2) presence of immunizing peptide and in 3) NIH 3T3 cell lysate. Samples were incubated in ORC1 polyclonal antibody (Product # PA5-23390) followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.

产品信息

PA5-23390

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1-3 µg/mL	-

产品规格
种属反应	Human, Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide (185 CQKPVRAKSKSAESPS 201) of ORC1L protein.
偶联物	Unconjugated
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Protein A
保存液	PBS with 0.05% BSA
内含物	0.05% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2540913

靶标信息

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。