Invitrogen

OSBP Polyclonal Antibody

OSBP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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OSBP Antibody (PA5-30110) in IHC (P)

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using OSBP (Product # PA5-30110) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

OSBP Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

OSBP Antibody — Effects of OSBP depletion on hPro-CpepSfGFP and CpepSfGFP. (A) OSBP immunostaining is concentrated in puncta that colocalize at the TGN with Golgin 97 (left) and are closely apposed to proinsulin (right). SfGFP mainly marks insulin granules and overlaps proinsulin (cyan) in young granules. Specificity of OSBP staining documented in Supplemental Figure S6. (B) Steady-state levels of both hPro-CpepSfGFP and CpepSfGFP are significantly decreased in OSBP knockdown cells as compared with control knockdown cells and are not restored by exogenous cholesterol. Quantification from Western blots; n = 4. (C) Endocytic uptake of lysosomal inhibitors partially restores both hPro-CpepSfGFP (hPro) and CpepSfGFP (Cpep) and increases the lysosomal SfGFP degradation product (Lys) in OSBP-depleted samples. The included image shows a representative Western blot (noncontiguous lanes are from the same blot). Quantification from Western blots; n = 4. (D) Images showing distinct separation of SfGFP fluorescence and Golgin97 immunostaining in control and ABCG1 knockdowns but increased overlap in OSBP knockdowns. Accompanying plot shows Manders’ overlap; control cells, # = 26; OSBP KD cells, # = 31. Data are presented as mean ± SEM; p values are determined by Student’s t test; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. {KD}

产品信息

PA5-30110

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 154 and 428 of Human OSBP

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.76 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2547584

产品详细信息

Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain.

Predicted reactivity: Mouse (97%), Rat (98%), Rabbit (97%), Rhesus Monkey (99%), Bovine (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。