Invitrogen

Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 488

Product Image Gallery

Occludin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Occludin Antibody, Alexa Fluor® 488 conjugate was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Occludin Antibody, Alexa Fluor® 488 conjugate (Product # 33-1511) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing cell junctional localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.

Occludin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Published figure using Occludin monoclonal antibody (Product # 331588) in Western Blot and Immunocytochemistry

Occludin Antibody in Western Blot (WB) — Figure 8 Inhibition of apelin receptor APJ with F13A dramatically reduces pathological vascular permeability in db/db mice via PI-3K/Akt and MAPK/Erk signaling pathways . The western blot results revealed that the expression of apelin receptor APJ (a), phosphorylation of cytoskeleton (VE-Cadherin and FAK) (b and c), tight junction (occludin) (d), and PI-3K/Akt and MEK/Erk signaling pathways proteins (e-h) were higher in diabetic retinopathy mice, compared with the F13A treated groups. Protein intensity was quantified by Image J software and expressed as fold of change relative to control (mean +- SD, n = 3). *** P < 0.001, ** P < 0.01, * P < 0.05 versus control one, Student's t -test. 1C: one-month control; 1M: one month treated with F13A, 3C: 3-month control; 3M: 3 months treated with F13A; 6C: 6-month control; 6M: 6 months treated with F13A; 9C: 9-month control; 9M: 9 months treated with F13A.

产品信息

331588

应用	建议稀释比	已发表文章
免疫印迹 (WB)	Assay-dependent	View 2 publications 2 publications
免疫组化 (IHC)	-	View 2 publications 2 publications
免疫组化（冰冻） (IHC (F))	-	View 2 publications 2 publications
免疫组织化学（悬浮细胞） (IHC (Free))	-	View 1 publication 1 publication
免疫细胞化学 (ICC/IF)	1-5 µg/mL	View 8 publications 8 publications
酶联免疫吸附实验 (ELISA)	Assay-dependent	-

产品规格
种属反应	Dog, Human, Mouse, Rat
已发表种属	Bovine, Dog, Human, Mouse
宿主/亚型	Mouse / IgG1, kappa
分类	Monoclonal
类型	Antibody
克隆号	OC-3F10
抗原	GST-fusion protein consisting of the C-terminal region (~150 aa) of human occludin.
偶联物	Alexa Fluor™ 488 Alexa Fluor 594 FITC HRP Unconjugated
激发/发射光谱	查看光谱
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Protein A
保存液	PBS, pH 7.4, with 4.0mg/mL BSA
内含物	0.1% sodium azide
保存条件	4° C, store in dark
RRID	AB_2532185

产品详细信息

Reactivity of this antibody with the occludin protein has been confirmed by immunofluorescence and western blotting (unconjugated). This antibody reacts specifically with mammalian occludin. Tissues/lysates that were tested with this antibody include T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma), MTE7B (Mouse), and rat liver.

靶标信息

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A human surfactant B deficiency air-liquid interface cell culture model suitable for gene therapy applications.

Molecular therapy. Methods & clinical development

Munis AM,Hyde SC,Gill DR

Published figure using Occludin monoclonal antibody (Product # 331588) in Immunocytochemistry

Fri Mar 12 00:00:00 EST 2021

A human surfactant B deficiency air-liquid interface cell culture model suitable for gene therapy applications.

Molecular therapy. Methods & clinical development

Munis AM,Hyde SC,Gill DR

Published figure using Occludin monoclonal antibody (Product # 331588) in Immunocytochemistry

Fri Mar 12 00:00:00 EST 2021

A Novel Three-Dimensional Glioma Blood-Brain Barrier Model for High-Throughput Testing of Tumoricidal Capability.

Frontiers in oncology

Sherman H,Rossi AE

Published figure using Occludin monoclonal antibody (Product # 331588) in Immunocytochemistry

Thu Oct 01 00:00:00 EDT 2020

A Novel Three-Dimensional Glioma Blood-Brain Barrier Model for High-Throughput Testing of Tumoricidal Capability.

Frontiers in oncology

Sherman H,Rossi AE

Published figure using Occludin monoclonal antibody (Product # 331588) in Immunocytochemistry

Thu Oct 01 00:00:00 EDT 2020

Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction.

Frontiers in bioengineering and biotechnology

Jing B,Wang ZA,Zhang C,Deng Q,Wei J,Luo Y,Zhang X,Li J,Du Y

Published figure using Occludin monoclonal antibody (Product # 331588) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction.

Frontiers in bioengineering and biotechnology

Jing B,Wang ZA,Zhang C,Deng Q,Wei J,Luo Y,Zhang X,Li J,Du Y

Published figure using Occludin monoclonal antibody (Product # 331588) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation.

Molecular human reproduction

DaSilva-Arnold SC,Kuo CY,Davra V,Remache Y,Kim PCW,Fisher JP,Zamudio S,Al-Khan A,Birge RB,Illsley NP

Fri Feb 01 00:00:00 EST 2019

Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways.

BioMed research international

Li Y,Bai YJ,Jiang YR,Yu WZ,Shi X,Chen L,Feng J,Sun GB

331588 was used in Immunofluorescence in immunohistochemistry on frozen tissues to investigate the effects and mechanism of apelin-13 in vascular permeability during diabetic macular oedema.

Mon Oct 01 00:00:00 EDT 2018

Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways.

BioMed research international

Li Y,Bai YJ,Jiang YR,Yu WZ,Shi X,Chen L,Feng J,Sun GB

331588 was used in Immunofluorescence in immunohistochemistry on frozen tissues to investigate the effects and mechanism of apelin-13 in vascular permeability during diabetic macular oedema.

Mon Oct 01 00:00:00 EDT 2018

Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways.

BioMed research international

Li Y,Bai YJ,Jiang YR,Yu WZ,Shi X,Chen L,Feng J,Sun GB

331588 was used in Immunofluorescence in immunohistochemistry on frozen tissues to investigate the effects and mechanism of apelin-13 in vascular permeability during diabetic macular oedema.

Mon Oct 01 00:00:00 EDT 2018

Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways.

BioMed research international

Li Y,Bai YJ,Jiang YR,Yu WZ,Shi X,Chen L,Feng J,Sun GB

331588 was used in Immunofluorescence in immunohistochemistry on frozen tissues to investigate the effects and mechanism of apelin-13 in vascular permeability during diabetic macular oedema.

Mon Oct 01 00:00:00 EDT 2018

Involvement of CYP1B1 in interferon γ-induced alterations of epithelial barrier integrity.

British journal of pharmacology

Alhouayek M,Gouveia-Figueira S,Hammarström ML,Fowler CJ

331588 was used in Immunohistochemistry-immunofluorescence to suggest that increased expression of CYP1B1 is involved in the effects of IFNγ on epithelial permeability.

Thu Mar 01 00:00:00 EST 2018

The EPAC-Rap1 pathway prevents and reverses cytokine-induced retinal vascular permeability.

The Journal of biological chemistry

Ramos CJ,Lin C,Liu X,Antonetti DA

331588 was used in Immunocytochemistry-Immunofluorescence to determine the role of the cAMP dependent guanine nucleotide exchange factor-Rap 1 pathway in retinal vascular permeability.

Fri Jan 12 00:00:00 EST 2018

The EPAC-Rap1 pathway prevents and reverses cytokine-induced retinal vascular permeability.

The Journal of biological chemistry

Ramos CJ,Lin C,Liu X,Antonetti DA

331588 was used in Immunocytochemistry-Immunofluorescence to determine the role of the cAMP dependent guanine nucleotide exchange factor-Rap 1 pathway in retinal vascular permeability.

Fri Jan 12 00:00:00 EST 2018

The EPAC-Rap1 pathway prevents and reverses cytokine-induced retinal vascular permeability.

The Journal of biological chemistry

Ramos CJ,Lin C,Liu X,Antonetti DA

331588 was used in Immunocytochemistry-Immunofluorescence to determine the role of the cAMP dependent guanine nucleotide exchange factor-Rap 1 pathway in retinal vascular permeability.

Fri Jan 12 00:00:00 EST 2018

Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1.

Proceedings of the National Academy of Sciences of the United States of America

Yanagida K,Liu CH,Faraco G,Galvani S,Smith HK,Burg N,Anrather J,Sanchez T,Iadecola C,Hla T

331588 was used in immunohistochemistry - free floating to describe a role for sphingosine 1-phosphate receptor-1 in regulating blood brain barrier permeability

Tue Apr 25 00:00:00 EDT 2017

Electro-Magnetic Nano-Particle Bound Beclin1 siRNA Crosses the Blood-Brain Barrier to Attenuate the Inflammatory Effects of HIV-1 Infection in Vitro.

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology

Rodriguez M,Kaushik A,Lapierre J,Dever SM,El-Hage N,Nair M

Published figure using Occludin monoclonal antibody (Product # 331588) in Immunocytochemistry

Wed Mar 01 00:00:00 EST 2017

Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier.

Nature communications

Campos Y,Qiu X,Gomero E,Wakefield R,Horner L,Brutkowski W,Han YG,Solecki D,Frase S,Bongiovanni A,d'Azzo A

331588 was used in immunohistochemistry - frozen section to analyze preservation of epithelial polarity and epithelial barrier by alix-mediated assembly of the actomyosin-tight junction polarity complex