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Invitrogen
OxPhos Blue Native WB Antibody Cocktail
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图: 1 / 11
OxPhos Blue Native WB Antibody (45-7999) in ICC/IF
Molecules and cells 2017 -
Fig. 2 PINK1/Parkin-independent changes in ROS levels by NAM treatment Levels of mitochondria content (A) and superoxide (B) were determined using NAO or DHE at Day 1 or 2 of NAM treatment, and the effects were compared between normal fibroblasts and those whose Parkin expression was knocked down by siRNA (siParkin). The image of the protein blot (top) shows diminished Parkin protein levels in cells treated... View More
产品信息
45-7999
产品规格
宿主/亚型
Mouse
/ Ig
分类
Cocktail
类型
Antibody
克隆号
Cocktail
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.5 mg/mL
保存条件
4°C, do not freeze
运输条件
Wet ice
RRID
产品详细信息
This Anti-OxPhos Complex Kit is a cocktail of monoclonal antibodies specific to human, bovine, mouse OxPhos Complex This antibody cocktail is validated for use in Western blotting.
Mouse monoclonal [20C11B11B11] to (C-I) NDUFA9:
Purification Method: IgG Fraction
Amount: 120 µg
Working Concentration: 2 µg/mL
Mouse monoclonal [2E3GC12FB2AE2] to (C-II-70) SDHA:
Purification Method: IgG Fraction
Amount: 6 µg
Working Concentration: 0.1 µg/mL
Mouse monoclonal [13G12AF12BB11] to (C-III-Core 2) UQCRC2:
Purification Method: IgG Fraction
Amount: 60 µg
Working Concentration: 1 µg/mL
Mouse monoclonal [20E8C12] to (C-IV-subunit IV) COX IV:
Purification Method: IgG Fraction
Amount: 60 µg
Working Concentration: 1 µg/mL
Mouse monoclonal [15H4C4 ] to (C-V-alpha) ATP5A:
Purification Method: Antigen Affinity Chromatography
Amount: 60 µg
Working Concentration: 1 µg/mL
靶标信息
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress. Blue Native polyacrylamide gel electrophoresis (BN-PAGE) is a simple and effective way to subfractionate mitochondrial proteins as intact complexes on a single gel (in one dimension). It can be used to detect altered assembly of these complexes arising from mutations in subunits, mutations in assembly factors, or mtDNA depletion. This type of analysis has been performed with biopsy samples, platelets and fibroblast cells from patients with suspected mitochondrial diseases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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