Invitrogen

P-Selectin Recombinant Rabbit Monoclonal Antibody (3H20L10)

P-Selectin Antibody in Immunocytochemistry (ICC/IF)

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P-Selectin Antibody (701257) in ICC/IF

Immunofluorescent analysis of P-selectin in U2OS cells using a P-selectin recombinant rabbit monoclonal antibody (Product # 701257) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing membrane localizatio... View More

P-Selectin Antibody in Western Blot (WB)

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产品信息

701257

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

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免疫细胞化学 (ICC/IF)

1:100-1:1,000

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酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

3H20L10

抗原

Peptide corresponding to amino acids 113-126 of human P-selectin

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532443

产品详细信息

This antibody is predicted to react with mouse, rat and non-human primate based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Carbon nano-onion-mediated dual targeting of P-selectin and P-glycoprotein to overcome cancer drug resistance.

Nature communications

Wang H,Liang Y,Yin Y,Zhang J,Su W,White AM,Xu J,Zhang Y,Stewart S,Lu X,He X

Published figure using P-Selectin recombinant monoclonal antibody (Product # 701257) in Immunocytochemistry

Tue Jan 12 00:00:00 UTC 2021

The cancer glycocalyx mediates intravascular adhesion and extravasation during metastatic dissemination.

Communications biology

Offeddu GS,Hajal C,Foley CR,Wan Z,Ibrahim L,Coughlin MF,Kamm RD

701257 was used in Simple Western - Size to further understand how tumour cells escape the vascular system to initiate metastatic sites.

Fri Feb 26 00:00:00 UTC 2021

生物信息学

蛋白别名: CD62 antigen-like family member P; CD62P; FLJ45155; GMP-140; Granule membrane protein 140; granule membrane protein 140kDa; granulocyte membrane protein; LECAM3; Leukocyte-endothelial cell adhesion molecule 3; P-selectin; PADGEM; Platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; RP1-86F14.2; selectin P (granule membrane protein 140kDa, antigen CD62)

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基因别名: CD62; CD62P; GMP140; GMRP; GRMP; LECAM3; PADGEM; PSEL; SELP

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UniProt ID: (Human) P16109

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Entrez Gene ID: (Human) 6403

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功能

lipopolysaccharide binding glycoprotein binding protein binding heparin binding sialic acid binding fucose binding glycosphingolipid binding calcium-dependent protein binding oligosaccharide binding

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