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Invitrogen
PACT Polyclonal Antibody
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PACT Antibody (PA5-96318) in WB
Western blot analysis of PACT in extracts of Mouse liver. Samples were incubated with PACT Polyclonal antibody (Product # PA5-96318) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-96318
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human PRKRA/PACT (NP_0036811)
偶联物
形式
Liquid
浓度
0.33 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MSQSRHRAEA PPLEREDSGT FSLGKMITAK PGKTPIQVLH EYGMKTKNIP VYECERSDVQ IHVPTFTFRV TVGDITCTGE GTSKKLAKHR AAEAAINILK ANASICFAVP DPLMPDPSKQ PKNQLNPIGS LQELAIHHGW RLPEYTLSQE GGPAHKREYT TICRLESFME TGKGASKKQA KRNAAEKFLA KFSNISPENH ISLTNVVGHS LGCTWHSLRN SPGEKINLLK RSLLSIPNTD YIQLLSEIAK EQGFNITYLD IDELSANGQY QCLAELSTSP ITVCHGSGIS CGNAQSDAAH NALQYLKIIA ERK; Positive Samples: Mouse liver; Cellular Location: Cytoplasm, perinuclear region
靶标信息
PRKRA appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. It activates EIF2AK2 in the absence of double-stranded RNA (dsRNA).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: interferon-inducible double stranded RNA-dependent protein kinase activator A; Interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; Protein activator of the interferon-induced protein kinase; protein kinase, interferon-inducible double stranded RNA-dependent activator; Protein kinase, interferon-inducible double-stranded RNA-dependent activator; RAX
基因别名: AV120107; lear; Pact; PRK; Prkra; RAX
UniProt ID: (Mouse) Q9WTX2
Entrez Gene ID: (Mouse) 23992
protein phosphorylation
positive regulation of cell proliferation
production of siRNA involved in RNA interference
gene silencing by RNA
pre-miRNA processing
cellular response to oxidative stress
production of miRNAs involved in gene silencing by miRNA
outer ear morphogenesis
middle ear morphogenesis
skeletal system morphogenesis
positive regulation of intrinsic apoptotic signaling pathway
