Invitrogen

PAH Polyclonal Antibody

PAH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PAH Antibody (PA5-79780) in IHC (P)

Immunohistochemistry (Paraffin) analysis of PAH in paraffin-embedded section of human liver cancer tissue using PAH Polyclonal Antibody (Product # PA5-79780). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution overnight at 4... View More

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产品信息

PA5-79780

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived human PAH recombinant protein (Position: R71-H208).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746895

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human HepG2 whole cell, human CACO-2 whole cell, rat kidney tissue, rat liver tissue, mouse kidney tissue, mouse liver tissue. IHC: human liver cancer tissue, mouse kidney tissue, rat kidney tissue. Flow: HepG2 cell.

靶标信息

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

仅用于科研。不用于诊断过程。未经明确授权不得转售。