Invitrogen

PAK4 Polyclonal Antibody

Name: PAK4 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-120128

查看其他12个PAK4抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

PAK4 Antibody in Immunocytochemistry (ICC/IF)

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PAK4 Antibody (PA5-120128) in ICC/IF

Immunofluorescence analysis of PAK4 in NIH/3T3 cells. Samples were incubated with PAK4 Polyclonal antibody (Product # PA5-120128) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

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Immunogen image

Immunogen Image PAK4 Polyclonal Antibody

产品信息

PA5-120128

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence within amino acids 450-550 of human PAK4 (NP_005875.1).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.65 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2913700

产品详细信息

Positive test controls include: LNCaP, Mouse testis, Mouse brain. The target is usually found in the following locations: Cytoplasm.

Immunogen sequence: HDGRVKLSDF GFCAQVSKEV PRRKSLVGTP YWMAPELISR LPYGPEVDIW SLGIMVIEMV DGEPPYFNEP PLKAMKMIRD NLPPRLKNLH KVSPSLKGFL D

靶标信息

PAK4 is a serine/ threonine p21 activating kinase implicated in the reorganization of actin cytoskeleton and in the formation of filopodia. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. The second group includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。