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ABS 006-24-02 detects native Pregnancy-associated plasma protein A (PAPP-A) from human samples. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.
ABS 006-24-02 has been successfully used in Western blotting, Immunohistochemistry and ELISA procedures. By Western blot, only unreduced forms of PAPA-A can be detected. In sandwich ELISA the optimal combination was BTE 004-09-02 as coating and biotinylated ABS 006-24-02 as detection. ABS 006-24-02 give the strongest singal in IHC compared to ABS 006-01-02 and BTE 004-09-02.
The epitope of ABS 006-24-02 is different from BTE 004-09-02 and ABS 006-01-02. The epitope is located on the PAPP-A subunit of the heterotetramer.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterottetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down's syndrome (trisomy 21).
Protein Aliases: aspecific BCL2 ARE-binding protein 2; differentially placenta 1 expressed protein; IGF-dependent IGFBP-4 protease; IGFBP-4 ase; insulin-like growth factor-dependent IGF binding protein-4 protease; Insulin-like growth factor-dependent IGF-binding protein 4 protease; PAPP A; PAPP-A; Pappalysin; Pappalysin-1; pregnacy-associated plasma protein A; Pregnancy-associated plasma protein A; pregnancy-associated plasma protein A, pappalysin 1; RP11-45A16.1
Gene Aliases: ASBABP2; DIPLA1; IGFBP-4ase; PAPA; PAPP-A; PAPPA; PAPPA1
UniProt ID: (Human) Q13219
Entrez Gene ID: (Human) 5069