Invitrogen

PARP1 Monoclonal Antibody (C.384.8)

PARP1 Antibody in Immunocytochemistry (ICC/IF)

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PARP1 Antibody (MA5-15031) in ICC/IF

Immunofluorescent analysis of PARP (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a PARP monoclonal antibody (Product # MA5-15031) at a dilution of 1:200 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1hr at room temperature. Nuclei (blue) were ... View More

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PARP1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of PARP using PARP Monoclonal Antibody (C.384.8) (Product # MA5-15031) shows increased expression of Cleaved PARP in HeLa and Jurkat cell line upon Staurosporine treatment. {TM}

产品信息

MA5-15031

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

查看5篇已发表文章 5篇已发表文章

免疫细胞化学 (ICC/IF)

1:800

免疫沉淀 (IP)

1:200

染色质免疫沉淀实验 (ChIP)

4 µL/10^6 cells

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

C.384.8

抗原

Synthetic peptide corresponding to residues surrounding Gly623 of human PARP-1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

438 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11000218

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with PARP-2 and PARP-3.

靶标信息

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Hypoxia deactivates epigenetic feedbacks via enzyme-derived clicking proteolysis-targeting chimeras.

Science advances

Do TC,Lau JW,Sun C,Liu S,Kha KT,Lim ST,Oon YY,Kwan YP,Ma JJ,Mu Y,Liu X,Carney TJ,Wang X,Xing B

MA5-15031 was used in Western Blotting to present an enzyme-derived clicking PROTACs (ENCTACs) capable of orthogonally cross-linking two disparate small-molecule warhead ligands that recognize BET bromodomain-containing protein 4 (BRD4) protein and E3 ligase within tumors only upon hypoxia-induced activation of nitroreductase enzyme.

Wed Dec 14 00:00:00 UTC 2022

Differential effects of poly(ADP ribose) polymerase inhibitor-based metronomic therapy on programmed death-ligand 1 and matrix-associated factors in human myeloid cells.

American journal of translational research

Ibba SV,Luu HH,Boulares AH

MA5-15031 was used in Western Blotting to show that PARPi-based metronomic therapy may promote functional changes in myeloid cells that provide an additional rationale for combining it with immunotherapy.

Thu Jan 12 00:00:00 UTC 2023

A Novel Role for the DNA Repair Enzyme 8-Oxoguanine DNA Glycosylase in Adipogenesis.

International journal of molecular sciences

Komakula SSB,Blaze B,Ye H,Dobrzyn A,Sampath H

Published figure using PARP1 monoclonal antibody (Product # MA5-15031) in Western Blot

Mon Jan 25 00:00:00 UTC 2021

Poly ADP Ribose Polymerase Inhibitor Olaparib Targeting Microhomology End Joining in Retinoblastoma Protein Defective Cancer: Analysis of the Retinoblastoma Cell-Killing Effects by Olaparib after Inducing Double-Strand Breaks.

International journal of molecular sciences

Jiang Y,Yam JC,Chu WK

MA5-15031 was used in Western Blotting to demonstrate the killing effects in retinoblastoma cells by PARP inhibitor olaparib after inducing DNA double-strand breaks.

Fri Oct 01 00:00:00 UTC 2021

Radiation induces EIF2AK3/PERK and ERN1/IRE1 mediated pro-survival autophagy.

Autophagy

Chaurasia M,Gupta S,Das A,Dwarakanath BS,Simonsen A,Sharma K

MA5-15031 was used in Western Blotting to investigate the relationship between radiation-induced oxidative stress, UPR and autophagy in murine macrophage cells.

Thu Aug 01 00:00:00 UTC 2019

生物信息学

蛋白别名: ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; Protein poly-ADP-ribosyltransferase PARP1

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基因别名: 5830444G22Rik; Adprp; ADPRT; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; pADPRT-1; PARP; PARP-1; PARP1; PPOL; sPARP-1

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UniProt ID: (Human) P09874, (Rat) P27008

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Entrez Gene ID: (Human) 142, (Rat) 25591, (Mouse) 11545

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功能

DNA binding DNA ligase (ATP) activity NAD+ ADP-ribosyltransferase activity protein binding transcription factor binding zinc ion binding enzyme binding protein kinase binding identical protein binding histone deacetylase binding poly(A) RNA binding protein N-terminus binding NAD binding R-SMAD binding transferase activity, transferring pentosyl groups chromatin binding transferase activity transferase activity, transferring glycosyl groups metal ion binding DNA metabolism protein

参与通路

negative regulation of transcription from RNA polymerase II promoter nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding double-strand break repair via homologous recombination lagging strand elongation DNA repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion double-strand break repair transcription from RNA polymerase II promoter protein ADP-ribosylation cellular response to DNA damage stimulus mitochondrion organization transforming growth factor beta receptor signaling pathway positive regulation of cardiac muscle hypertrophy protein autoprocessing protein sumoylation signal transduction involved in regulation of gene expression macrophage differentiation mitochondrial DNA metabolic process cellular response to insulin stimulus nucleotide-excision repair, DNA incision cellular response to oxidative stress protein modification process DNA damage response, detection of DNA damage mitochondrial DNA repair positive regulation of transcription from RNA polymerase II promoter DNA ligation involved in DNA repair positive regulation of SMAD protein import into nucleus protein poly-ADP-ribosylation global genome nucleotide-excision repair regulation of cellular protein localization negative regulation of telomere maintenance via telomere lengthening ATP generation from poly-ADP-D-ribose positive regulation of transcription regulatory region DNA binding telomere maintenance DNA metabolic process base-excision repair transcription, DNA-templated regulation of growth rate cellular response to superoxide regulation of transcription, DNA-templated behavioral response to cocaine regulation of single strand break repair

PARP1 Monoclonal Antibody (C.384.8)

PARP1 Antibody (MA5-15031) in ICC/IF

产品信息

MA5-15031

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

PARP1 Monoclonal Antibody (C.384.8)

PARP1 Antibody (MA5-15031) in ICC/IF

产品信息

MA5-15031

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)