Invitrogen

PAX6 Monoclonal Antibody (13B10-1A10)

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10个已发表图片
22 篇参考文献
查看其他43个PAX6抗体

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产品信息

MA1-109

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:2,000
查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

-
查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200
-

免疫组化（冰冻） (IHC (F))

Assay-dependent
查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:200
查看10篇已发表文章 10篇已发表文章

流式细胞分析 (Flow)

1:100
查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

-
查看1篇已发表文章 1篇已发表文章
产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

13B10-1A10

抗原

Purified internal fragment of human recombinant PAX6 expressed in E. coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2536820

产品详细信息

Western blot analysis of MA1-109 detects an ~47 kDa protein in whole cell lysates from early passage human B3 lens epithelial cells and in mouse eye lysate. An additional unknown band at ~36 kDa is also detected in B3 cell lysates. This antibody will detect additional nonspecific bands in lysates with very low basal PAX6 expression. Specificity of the antibody was confirmed in B3 and mouse eye lysates (positive controls) compared to U-2 OS human osteosarcoma and NIH/3T3 mouse embryonic fibroblasts (negative controls).

MA1-109- can be used for immunofluoresence and flow cytometry analysis of Pax6 in human neural stem cells.

靶标信息

This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Aniridia; Aniridia type II protein; Dickie's small eye; Keratitis; MGC17209; Oculorhombin; paired box gene 6; Paired Box Gene-6; paired box homeotic gene-6; Paired box protein Pax-6; small eye

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基因别名: 1500038E17Rik; AEY11; AN; AN2; D11S812E; Dey; FVH1; Gsfaey11; MGDA; Pax-6; PAX6; Sey; WAGR

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UniProt ID: (Human) P26367, (Mouse) P63015

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Entrez Gene ID: (Human) 5080, (Mouse) 18508

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功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding ubiquitin-protein transferase activity protein binding transcription factor binding protein kinase binding ubiquitin protein ligase binding histone acetyltransferase binding co-SMAD binding R-SMAD binding HMG box domain binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding AT DNA binding double-stranded DNA binding sequence-specific DNA binding transcription regulatory region DNA binding
参与通路
negative regulation of transcription from RNA polymerase II promoter establishment of mitotic spindle orientation blood vessel development eye development cell fate determination neuron migration negative regulation of protein phosphorylation positive regulation of neuroblast proliferation lens development in camera-type eye type B pancreatic cell differentiation pancreatic A cell development transcription from RNA polymerase II promoter smoothened signaling pathway axon guidance central nervous system development salivary gland morphogenesis visual perception response to wounding regulation of asymmetric cell division organ morphogenesis dorsal/ventral axis specification positive regulation of gene expression protein ubiquitination oligodendrocyte cell fate specification cerebral cortex regionalization forebrain anterior/posterior pattern specification forebrain dorsal/ventral pattern formation commitment of neuronal cell to specific neuron type in forebrain forebrain-midbrain boundary formation regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification regulation of transcription from RNA polymerase II promoter involved in somatic motor neuron fate commitment pituitary gland development signal transduction involved in regulation of gene expression keratinocyte differentiation regulation of cell migration positive regulation of epithelial cell differentiation lacrimal gland development protein localization to organelle eye photoreceptor cell development glucose homeostasis negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of timing of cell differentiation embryonic camera-type eye morphogenesis neuron fate commitment astrocyte differentiation negative regulation of epithelial cell proliferation negative regulation of neurogenesis retina development in camera-type eye iris morphogenesis cornea development in camera-type eye negative regulation of neural precursor cell proliferation neural crest cell migration epithelial cell development pancreatic A cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development axonogenesis brain development negative regulation of cell proliferation anterior/posterior pattern specification dorsal/ventral pattern formation regulation of gene expression regulation of neuron projection development pallium development rhombomere morphogenesis olfactory bulb development telencephalon regionalization interkinetic nuclear migration cell differentiation forebrain development hindbrain development positive regulation of cell fate specification camera-type eye development cell fate commitment regulation of neuron differentiation regulation of neurogenesis neural precursor cell proliferation positive regulation of glucagon secretion insulin metabolic process positive regulation of neuron migration
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