Invitrogen

PCAF Recombinant Rabbit Monoclonal Antibody (8W6M0)

查看其他27个PCAF抗体

问题与答案

问题与答案

问题与答案

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抗体检测数据 (8)

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PCAF Antibody (MA568918) in IHC (P)

Immunohistochemistry analysis of PCAF on paraffin-embedded Human pancreas tissue. Samples were incubated with PCAF recombinant monoclonal antibody (Product # MA568918) using a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0).

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCAF Antibody in Western Blot (WB)

PCAF Antibody in Immunoprecipitation (IP)

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PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PCAF on paraffin-embedded Human pancreas tissue. Samples were incubated with PCAF recombinant monoclonal antibody (Product # MA568918) using a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0).

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PCAF on paraffin-embedded Human thyroid tissue. Samples were incubated with PCAF recombinant monoclonal antibody (Product # MA568918) using a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0).

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PCAF on paraffin-embedded Mouse kidney tissue. Samples were incubated with PCAF recombinant monoclonal antibody (Product # MA568918) using a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0).

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PCAF on paraffin-embedded Mouse colon tissue. Samples were incubated with PCAF recombinant monoclonal antibody (Product # MA568918) using a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0).

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PCAF on paraffin-embedded Rat kidney tissue. Samples were incubated with PCAF recombinant monoclonal antibody (Product # MA568918) using a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0).

PCAF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PCAF on paraffin-embedded Rat colon tissue. Samples were incubated with PCAF recombinant monoclonal antibody (Product # MA568918) using a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0).

PCAF Antibody in Western Blot (WB) — Western blot analysis of PCAF in HepG2 and Jurkat cells. Samples were probed with PCAF recombinant monoclonal antibody (Product # MA568918) at 1:1,000 dilution incubated at room temperature for 1. 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

PCAF Antibody in Immunoprecipitation (IP) — Immunoprecipitation analysis of PCAF was performed using 2 µg of PCAF recombinant monoclonal antibody (Product # MA568918). Rabbit IgG isotype control was used to precipitate the Control IgG sample. Western blot analysis of immunoprecipitates was conducted using PCAF at a 1:1,000 dilution.

产品信息

MA568918

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:4,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:2,000

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

免疫沉淀 (IP)

0.5-4 µg

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

8W6M0

抗原

Recombinant protein (or fragment).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.74 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3., with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

PCAF (histone acetyltransferase KAT2B) functions as a histone acetyltransferase (HAT) to promote transcriptional activation. It has significant histone acetyltransferase activity with core histones (H3 and H4) and nucleosome core particles. PCAF also acetylates non-histone proteins such as ACLY, PLK4, and TBX5. It inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. PCAF also acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It is involved in heart and limb development by mediating acetylation of TBX5 and acetylation regulating nucleocytoplasmic shutting of TBX5.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CREBBP-associated factor; Histone acetylase PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; K(lysine) acetyltransferase 2B; Lysine acetyltransferase 2B; P/ PCAF; P/CAF; P300/CBP-associated factor; Spermidine acetyltransferase KAT2B

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基因别名: A930006P13Rik; AI461839; AW536563; CAF; KAT2B; P/CAF; PCAF

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UniProt ID: (Human) Q92831, (Mouse) Q9JHD1

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Entrez Gene ID: (Human) 8850, (Rat) 301164, (Mouse) 18519

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RNA polymerase II regulatory region sequence-specific DNA binding chromatin binding transcription cofactor activity transcription coactivator activity diamine N-acetyltransferase activity histone acetyltransferase activity lysine N-acetyltransferase activity, acting on acetyl phosphate as donor cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding enzyme activator activity H3 histone acetyltransferase activity acetyltransferase activity transferase activity transferase activity, transferring acyl groups transferase activity, transferring acyl groups other than amino-acyl groups protein kinase binding histone acetyltransferase binding histone deacetylase binding histone acetyltransferase activity (H3-K9 specific) peptide-lysine-N-acetyltransferase activity DNA-binding transcription factor binding transcription factor binding protein complex binding N-acetyltransferase activity

chromatin remodeling protein acetylation memory negative regulation of cell proliferation regulation of protein ADP-ribosylation positive regulation of neuron projection development histone acetylation N-terminal peptidyl-lysine acetylation internal peptidyl-lysine acetylation peptidyl-lysine acetylation cellular response to insulin stimulus cellular response to oxidative stress positive regulation of chromatin binding positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter histone H3 acetylation histone H3-K9 acetylation negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of transcription, DNA-templated positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter cellular response to parathyroid hormone stimulus positive regulation of histone H3-K14 acetylation positive regulation of histone H3-K9 acetylation negative regulation of transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase II promoter by glucose gluconeogenesis regulation of DNA repair regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter ubiquitin-dependent protein catabolic process fatty acid biosynthetic process heart development regulation of gene expression vasodilation regulation of RNA splicing negative regulation of fatty acid biosynthetic process positive regulation of gluconeogenesis positive regulation of fatty acid biosynthetic process positive regulation of gene expression, epigenetic positive regulation of glycolytic process regulation of embryonic development negative regulation of centriole replication positive regulation of lipid biosynthetic process rhythmic process regulation of cell division attachment of mitotic spindle microtubules to kinetochore regulation of cell cycle limb development regulation of small molecule metabolic process negative regulation of ferroptosis positive regulation of attachment of mitotic spindle microtubules to kinetochore negative regulation of rRNA processing transcription, DNA-templated cell cycle