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PDGFRA Antibody (701142) in ICC/IF
Immunofluorescent analysis of PDGFR alpha in HeLa cells using a PDGFR alpha recombinant rabbit monoclonal antibody (Product # 701142) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic and nuclear membrane localization of PDGFR-alpha.
产品信息
701142
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
7H13L1
抗原
Recombinant protein corresponding to amino acids 259-436 of human platelet-derived growth factor receptor - alpha polypeptide
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532408
产品详细信息
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; CD140a antigen; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF Receptor alpha; PDGF-R-alpha; PDGFR-2; pdgfr-a; PDGFRA/BCR fusion; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; Platelet-derived growth factor receptor alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein
基因别名: CD140A; GAS9; PDGFR-2; PDGFR2; PDGFRA; RHEPDGFRA
UniProt ID: (Human) P16234
Entrez Gene ID: (Human) 5156
protein kinase activity
transmembrane receptor protein tyrosine kinase activity
platelet-derived growth factor alpha-receptor activity
vascular endothelial growth factor-activated receptor activity
Ras guanyl-nucleotide exchange factor activity
platelet-derived growth factor receptor binding
protein binding
ATP binding
vascular endothelial growth factor binding
protein homodimerization activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
platelet-derived growth factor binding
transmembrane signal receptor
MAPK cascade
luteinization
cell activation
positive regulation of cytosolic calcium ion concentration
positive regulation of cell proliferation
negative regulation of platelet activation
positive regulation of phospholipase C activity
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
viral process
peptidyl-tyrosine phosphorylation
positive regulation of cell migration
cellular response to reactive oxygen species
platelet-derived growth factor receptor-alpha signaling pathway
positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
wound healing
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of DNA replication
protein autophosphorylation
phosphatidylinositol phosphorylation
platelet-derived growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of fibroblast proliferation
embryonic digestive tract morphogenesis
embryonic cranial skeleton morphogenesis
embryonic skeletal system morphogenesis
regulation of chemotaxis
cardiac myofibril assembly
cell chemotaxis
retina vasculature development in camera-type eye
positive regulation of ERK1 and ERK2 cascade
platelet aggregation
metanephric glomerular capillary formation
regulation of actin cytoskeleton reorganization
regulation of mesenchymal stem cell differentiation
