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Invitrogen
PDHA1A Polyclonal Antibody
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PDHA1A Antibody (PA5-72684) in WB
Western blot analysis of PDHA1A in various lysates. Samples were incubated with PDHA1A polyclonal antibody (Product # PA5-72684) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Zebrafish lysate; Lane 2: ZF4 whole cell lysate. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
产品信息
PA5-72684
产品规格
种属反应
Zebrafish
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH conjugated synthetic peptide between 233~263 amino acids from the Central region of Zebrafish PDHA1
偶联物
形式
Liquid
浓度
0.97 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: pdha1b; PDHE1-A type I; PHE1A; pyruvate dehydrogenase (lipoamide) alpha 1; pyruvate dehydrogenase (lipoamide) alpha 1a; pyruvate dehydrogenase E1 alpha 1 subunit a
基因别名: im:6895726; pdha1; wu:fd18b01; wu:fo96f03; zgc:73271; zgc:86692
Entrez Gene ID: (Zebrafish) 406702
