Invitrogen

PDPK1 Polyclonal Antibody

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PDPK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PDPK1 on paraffin-embedded human lung cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with PDPK1 polyclonal antibody (Product# PA5-79801) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

PDPK1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of PDPK1 on mouse small intestine tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with PDPK1 polyclonal antibody (Product# PA5-79801) with a dilution of 1 µg/mL (overnight at 4°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

PDPK1 Antibody in Western Blot (WB) — Western blot analysis of PDPK1 in Lane 1: rat liver tissue lysate, Lane 2: rat lung tissue lysate, Lane 3: mouse liver tissue lysate, Lane 4: mouse lung tissue lysate, Lane 5: COLO320 whole cell lysate, Lane 6: MCF-7 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with PDPK1 polyclonal antibody (Product # PA5-79801) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

产品信息

PA5-79801

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.1-0.5 µg/mL	-
免疫组化（石蜡） (IHC (P))	0.5-1 µg/mL	-
免疫组化（冰冻） (IHC (F))	0.5-1 µg/mL	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	A synthetic peptide corresponding to a sequence at the C-terminus of human PDPK1.
偶联物	Unconjugated
形式	Lyophilized
浓度	500 µg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS with 5mg BSA
内含物	0.05mg sodium azide
保存条件	-20°C
RRID	AB_2746916

产品详细信息

The synthetic peptide sequence is 524-556aa, YLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

靶标信息

PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; HGNC:8809; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; mPDK1; OTTHUMP00000174525; OTTHUMP00000205076; PkB kinase; PkB kinase like gene 1; PkB-like 1; Protein kinase B kinase

基因别名: PDK1; PDPK1; PDPK2; PDPK2P; PRO0461

UniProt ID: (Human) O15530, (Mouse) Q9Z2A0, (Rat) O55173

Entrez Gene ID: (Human) 5170, (Mouse) 18607, (Rat) 81745

分子生物学功能: non-receptor serine/threonine protein kinase