Invitrogen

PEF1 Polyclonal Antibody

Name: PEF1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-114395
Availability: InStock

查看其他9个PEF1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (6)

PEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 6

PEF1 Antibody (PA5-114395) in IHC (P)

Immunohistochemistry analysis of PEF1 in paraffin-embedded human colon cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PEF1 polyclonal antibody (Product # PA5-114395) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen Image PEF1 Polyclonal Antibody

产品信息

PA5-114395

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human PEF1 recombinant protein (Position: R170-L284).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2884851

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human HEK293 whole cell, human placenta tissue, human PC-3 whole cell, human U-87MG whole cell, human U-937 whole cell, human HepG2 whole cell, human A431 whole cell, human U2OS whole cell, rat lung tissues, rat testicular tissue, mouse lung tissue, mouse testicular tissue. IHC: mouse testis tissue, rat testis tissue, human colon cancer tissue, human lung cancer tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1), sorcin (SRI), grancalcin (GCA), and ALG2. PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1), sorcin (SRI), grancalcin (GCA), and ALG2 (PDCD6) (Kitaura et al., 2001 ).

仅用于科研。不用于诊断过程。未经明确授权不得转售。