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Agrisera
PELOTA/Protein pelota homolog Polyclonal Antibody
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PELOTA/Protein pelota homolog Antibody (AS16-3984) in WB
10 µg of total protein from Solanum lycopersicum overexpressing PELOTA GFP leaf extracted by SDS buffer and denatured at 100°C for 2-5 min. Proteins were separated on 10% SDS-PAGE minigel and blotted 1 h to PVDF using semi-dry or tank transfer. Blots were blocked with for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 for 1 h at RT with agita... View More
产品信息
AS16-3984
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant PELOTA protein of Solanum lycopersicum, full length.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Solanum lycopersicum
靶标信息
PELOTA is a ribosome rescue factor in plants that resolves stalled translation complexes to maintain protein synthesis and mRNA quality control. It recognizes ribosomes arrested on problematic mRNAs, often at no stop or damaged transcripts, and works with HBS1 and ABCE1 to promote ribosome splitting, release of peptidyl tRNA, and targeting of aberrant mRNAs for decay through pathways such as nonstop and no go decay. By sustaining translational throughput and preventing accumulation of defective proteins, PELOTA supports growth, stress tolerance, and development, with expression and activity adjusting under heat, oxidative stress, and nutrient limitation. Plant PELOTA also influences organelle biogenesis and hormone responsive transcription indirectly via its effects on translational homeostasis, and genetic perturbation leads to defects in meristem activity, fertility, and resilience due to impaired ribosome quality control.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT4G27650; PEL1
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