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Invitrogen
PEMT Polyclonal Antibody
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PEMT Antibody (PA5150350) in WB
Western blot analysis of PEMT in various lysates. Samples were incubated with PEMT polyclonal antibody (Product # PA5150350) using a dilution of 1:4,000 in 5% NFDM/TBST at 4°C overnight followed by Goat Anti-Rabbit IgG - HRP Secondary Antibody at a dilution of 1:50,000 for 1 hour at room temperature. Lane 1: Mouse liver tissue lysate; Lane 2: Mouse colon tissue lysate; Lane 3: Rat testis tissue lysate. Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa; Observed band size: 30 kDa. Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAG... View More
产品信息
PA5150350
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide within mouse PEMT aa 67-93.
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Positive control: Mouse liver tissue lysate, Mouse colon tissue lysate, Rat testis tissue lysate.
Subcellular location: Endoplasmic reticulum membrane, Mitochondrion membrane.
靶标信息
This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: MGC2483; PE N-methyltransferase; PEAMT; Phosphatidylethanolamine N-methyltransferase; Phospholipid methyltransferase; PLMT
基因别名: PEAMT; Pempt; Pempt2; Pemt; PEMT2; PHOMETH
UniProt ID: (Rat) Q08388, (Mouse) Q61907
Entrez Gene ID: (Rat) 25511, (Mouse) 18618
phosphatidyl-N-methylethanolamine N-methyltransferase activity
phosphatidylethanolamine N-methyltransferase activity
N-methyltransferase activity
phosphatidylethanolamine binding
S-adenosylmethionine-dependent methyltransferase activity
phosphatidyl-N-dimethylethanolamine N-methyltransferase activity
methyltransferase activity
transferase activity
methyltransferase
transferase
metabolite interconversion enzyme
phospholipid metabolic process
glycerophospholipid metabolic process
phosphatidylcholine biosynthetic process
response to nutrient
negative regulation of cell proliferation
methylation
response to vitamin
response to drug
response to amino acid
response to ethanol
S-adenosylhomocysteine metabolic process
S-adenosylmethionine metabolic process
positive regulation of lipoprotein metabolic process
lipid metabolic process
phospholipid biosynthetic process
positive regulation of protein targeting to mitochondrion
