Invitrogen

PER1 Polyclonal Antibody

Name: PER1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-524

高级验证

6个已发表图片

24 篇参考文献

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说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

PER1 Antibody in Immunocytochemistry (ICC/IF)

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PER1 Antibody (PA1-524) in ICC/IF

Immunofluorescent analysis of PER1 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PER1 polyclonal antibody (Product # PA1-524) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyL... View More

PER1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PER1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PER1 Antibody — Fig. 4 Knockdown of PER1 in the dorsal hippocampus impairs long-term memory. Per1 mRNA expression is upregulated 60 m in the young hippocampus after a OLM ( t (8) = 4.49, ** p < 0.01; n = 5, 5; all male) and b context fear conditioning ( t (8) = 3.01, *p < 0.05, n = 5, 5; all male). c Top, schematic of ChIP primer sites along the Per1 promoter. Bottom, HDAC3 ChIP results. HDAC3 occupancy was reduced along the Per1 promoter in the young hippocampus after OLM (Training effect only, F (1,21) = 8.51, ** p < 0.01, n = 4, 5; all male). d Hippocampal PER1 protein expression was significantly reduced by Per1 siRNA infusion ( t (20) = 2.72, * p = 0.01, n = 11,11; all male). e Per1 knockdown impaired OLM (Two-way ANOVA: siRNA x Session ( F (1,19) = 29.22, p < 0.001), Sidak's post hoc tests, *** p < 0.001, n = 11, 10; all male). f Per1 siRNA did not affect total exploration time at test ( t (19) = 0.27, p = 0.79). Data are presented as mean +- SEM {KD}

Immunogen image

Immunogen Image PER1 Polyclonal Antibody

产品信息

PA1-524

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

查看11篇已发表文章 11篇已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:50-1:500

免疫组化（冰冻） (IHC (F))

1:50-1:500

免疫细胞化学 (ICC/IF)

1:10-1:200

查看3篇已发表文章 3篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

Fruit fly, Hamster, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: P(39) S L A D D T D A N S N G(51) C

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2268030

产品详细信息

PA1-524 detects PER1 from human and mouse tissues as well as recombinant mouse PER1.

PA1-524 has been sucessfully used in Western blot, immunofluoresence and immunohistochemistry procedures. By Western blot, this antibody detects an ~136 kDa protein representing overexpressed recombinant mouse PER1 protein. Immunofluorescence staining of PER1 in mouse testes with PA1-524 results in cytoplasmic staining.

PA1-524 immunizing peptide corresponds to amino acid residues 39-51 from mouse PER1. PA1-524 immunizing peptide (Cat. # PEP-076) is available for use in neutralization and control experiments.

靶标信息

Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Circadian clock protein PERIOD 1; Circadian pacemaker protein Rigui; hPER1; mPER1; Period circadian protein homolog 1; period homolog 1; Period, drosophila, homolog of

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基因别名: hPER; KIAA0482; m-rigui; mPer1; PER; PER1; RIGUI

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UniProt ID: (Human) O15534, (Mouse) O35973

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Entrez Gene ID: (Human) 5187, (Mouse) 18626

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功能