Invitrogen

PGK1 Polyclonal Antibody

Name: PGK1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-13973

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1个已发表图片

1 篇参考文献

查看其他17个PGK1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

说明书

实验方案

技术支持

PGK1 Antibody in Immunohistochemistry (IHC)

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PGK1 Antibody (PA5-13973) in IHC

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using a PGK1 polyclonal antibody (Product # PA5-13973), followed by HRP-conjugated secondary antibody and DAB staining.

Product Image Gallery

PGK1 Antibody — Figure 3 Downregulation of glycolytic regulatory enzymes expression through TGFbeta2 signaling to promote pro-survival mitochondrial-priming 3A. Induction of autocrine TGFbeta2 cytokine expression in HCC827 cells persisted after 9 days of erlotinib treatment undergoing adaptive drug-escape, correlated with downregulated expression of the key glucose metabolism regulatory enzymes GPI , PGK1 and ENO2 , as well as the cell cycle progression regulator TIMELESS . (a): HCC827 cells were treated without (0 hr) or with erlotinib for 8 hr, 9 days, and 9 days of TKI followed by 7 days of drug washout. Actin (bottom panel) was included as loading control. (b): Gene expression heat map signature showing adaptive suppression in gene expression of glucose metabolism regulatory Warburg genes GPI , PGK1 and ENO2 in HCC827 cells ( upper panel ) treated with erlotinib and H1975 cells ( lower panel ) treated with CL-387,785. TKI treatment conditions: a, 0 hr (untreated control); b, 8 hr; c , 9 days and d , 9 days of TKI followed by 7 days of drug washout. 3B. Immunoblot analysis of the effect of TGFbeta2 on HCC827 and PC-9 cells in the expression levels of the glucose metabolism regulatory enzymes: GPI, PGK1, ENO2, and the mitochondrial prosurvival marker BCL-2/BCL-xL. (a) HCC827 cells were treated with exogenous TGFbeta2 (5 ng/ml) for 9 days, at increasing concentration of the cytokine as indicated for immunoblotting. (b & c) HCC827 and PC-9 cells under similar treatment conditions as above we {TM}

产品信息

PA5-13973

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:50

产品规格

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 305-334 amino acids from the central region of human PGK1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Lot-specific

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2161229

产品详细信息

This antibody is predicted to react with non-human primate based on sequence homology.

靶标信息

Also known as ATP:3-phosphoglycerate 1-phosphotransferase showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

PGK1 Polyclonal Antibody

PGK1 Antibody (PA5-13973) in IHC

产品信息

PA5-13973

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

保存条件

运输条件

RRID

产品详细信息

靶标信息

性能保障

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