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Invitrogen
PHAPI2 Polyclonal Antibody
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FIGURE: 1 / 2
PHAPI2 Antibody (PA5-101400) in ICC/IF
Immunocytochemistry analysis of PHAPI2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PHAPI2 polyclonal antibody (Product # PA5-101400) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-101400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ANP32B(Accession Q92688), corresponding to amino acid residues D219-D251.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ANP32B.
Target Information
Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acidic leucine-rich nuclear phosphoprotein 32 family member B; acidic nuclear phosphoprotein 32 family, member B; Acidic protein rich in leucines; OTTHUMP00000063770; PHAPI2; proliferation related acidic leucine rich protein PAL31; Proliferation-related acidic leucine-rich protein PAL31; Putative HLA-DR-associated protein I-2; Silver-stainable protein SSP29; TNF superfamily member 13
Gene Aliases: 2410015B15Rik; ANP32B; APRIL; PAL31; PHAPI2; PHAPI2a; Ssp29
UniProt ID: (Mouse) Q9EST5, (Rat) Q9EST6
Entrez Gene ID: (Mouse) 67628, (Rat) 170724
G1/S transition of mitotic cell cycle
vasculature development
positive regulation of cell proliferation
ventricular system development
inner ear development
palate development
positive regulation of G1/S transition of mitotic cell cycle
nucleosome assembly
activation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of cell differentiation
positive regulation of protein export from nucleus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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