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Invitrogen
PIF4 Polyclonal Antibody
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PIF4 Antibody (PA5-120777) in WB
Western blot analysis of PIF4 in extracts of various cell lines. Samples were incubated with PIF4 Polyclonal antibody (Product # PA5-120777) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
PA5-120777
产品规格
种属反应
Plant
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide.
偶联物
形式
Liquid
浓度
1.43 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive test controls include: seedling, rosette leaf.
Immunogen sequence: IDYLKSLQLQ LQVMWMGSGM AAAAASAPMM FPGVQPQQFI RQIQSPVQLP RFPVMDQSAI QNNPGLVCQN PVQNQIISDR FARYIGGFPH MQAATQMQPM E
靶标信息
Transcription factor acting negatively in the phytochrome B signaling pathway. May regulate the expression of a subset of genes involved in cell expansion by binding to the G-box motif. Activated by CRY1 and CRY2 in response to low blue light (LBL) by direct binding at chromatin on E-box variant 5'-CA[CT]GTG-3' to stimulate specific gene expression to adapt global physiology (e.g. hypocotyl elongation in low blue light). [Uniprot]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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