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Invitrogen
PIM1 Recombinant Polyclonal Antibody (19HCLC)
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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PIM1 Antibody (710504) in ICC/IF
Immunofluorescence analysis of Serine/threonine-protein kinase pim-1 was performed using 70 confluent log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PIM1 Recombinant Polyclonal Antibody (19HCLC) (Product # 710504) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, ... View More
产品信息
710504
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Polyclonal
类型
Antibody
克隆号
19HCLC
抗原
Peptide corresponding to amino acids 115-130 of human PIM-1
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532727
产品详细信息
This antibody is predicted to react with bovine, canine, equine, feline, non-human primate, mouse, rabbit, ovine and porcine based on sequence homology.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
The proto-oncogene PIM1 is upregulated in prostate cancer. PIM1, which belongs to the Serine/Threonine protein kinase family, is thought to play a role in signal transduction in blood cells. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer. It may affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. PIM1 is expressed primarily in cells of the hematopoietic and germ line lineages.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase pim-1; Serine/threonine-protein kinase pim-1
基因别名: PIM; Pim-1; PIM1
UniProt ID: (Human) P11309, (Mouse) P06803
Entrez Gene ID: (Human) 5292, (Mouse) 18712
protein phosphorylation
apoptotic process
cell cycle
multicellular organism development
cell proliferation
hyaluronan metabolic process
negative regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
protein autophosphorylation
vitamin D receptor signaling pathway
regulation of mitotic cell cycle
positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle
