Description: This 10-1-PIR monoclonal antibody reacts with mouse paired Ig-like receptors of activating (PIR)-A and -B. These cell surface glycoproteins, which contain six extracellular Ig-like domains with distinct transmembrane and cytoplasmic regions, are expressed together on B lymphocytes, dendritic cells, macrophages, granulocytes, platelets, and mast cells. However, expression of PIR-A/B has not been observed on T cells, NK cells, and erythrocytes. The PIR-A receptor interacts with signaling molecules containing immunoreceptor tyrosine-based activation motifs (ITAMs) which lead to its activating function. In contrast, the PIR-B receptor associates with proteins possessing immunoreceptor tyrosine-based inhibitory motifs (ITIMs); therefore, this receptor has been shown to have an inhibitory function. PIRs bind MHC class I to modulate cell signaling and homeostasis of the immune system. Moreover, PIR-B knockout mice have been shown to exhibit susceptibility to Salmonella infection.
Crossblocking studies indicate that 10-1-PIR recognizes a different epitope from 6C1 (cat. no. 46-5978).
Applications Reported: This 10-1-PIR antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 10-1-PIR antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Paired Ig-like receptors of activating (PIR)-A and -B are expressed on B lymphocytes, macrophages, dendritic cells, platelets, granulocytes, and mast cells, but not on T cells, NK cells, and erythrocytes. These cell surface glycoproteins contain six extracellular Ig-like domains with distinct cytoplasmic and transmembrane regions. The PIR-A receptor activates through signaling molecules containing immunoreceptor tyrosine-based activation motifs. In contrast, the PIR-B receptor inhibits through proteins possessing immunoreceptor tyrosine-based inhibitory motifs. PIRs bind MHC class I to modulate immune system homeostasis and cell signaling.
蛋白别名: Cell-surface glycoprotein p91; Gp49b; leukocyte immunoglobulin-like receptor 3; Leukocyte immunoglobulin-like receptor subfamily B member 3; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3; Lilrb4; LIR-3; paired immunoglobulin-like receptor; Paired immunoglobulin-like receptor B; paired-Ig-like receptor A1; PIR-B
基因别名: 6M21; Gp91; Lilrb3; LIR-3; Ly89; Pir; PIR-A1; PIR-B; Pirb
UniProt ID: (Mouse) P97484
分子生物学功能: immunoglobulin receptor superfamily