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PIWIL1 Antibody (701177) in ICC/IF
Immunofluorescence analysis of Piwi-like protein 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PIWIL1 Recombinant Rabbit Monoclonal Antibody (10H15L2) (Product # 701177) at a concentration of 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Sec... View More
产品信息
701177
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
10H15L2
抗原
Recombinant protein corresponding to amino acids 90-213 of human Hiwi
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532421
产品详细信息
This antibody is predicted to react with mouse based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Hiwi is a member of the Piwi /Argonaute protein family. Hiwi is specifically expressed in germline cells such as spermatocytes and round spermatids during spermatogenesis. Hiwi is also expressed in CD34+ hematopoietic progenitors, but not in CD34- hematopoietic cells, suggesting a role for this gene in the maintenance of hematopoietic stem cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: HILI; mili; Miwi like; Miwi likw; piwi homolog; piwi like homolog 1; piwi-like 1; piwi-like homolog 1; Piwi-like protein 1; piwi-like protein 2; piwi-like RNA-mediated gene silencing 1; PIWIL1L; piwil2-like protein
基因别名: CT80.1; HIWI; MIWI; PIWI; PIWIL1
UniProt ID: (Human) Q96J94, (Mouse) Q9JMB7
Entrez Gene ID: (Human) 9271, (Rat) 363912, (Mouse) 57749
