Invitrogen

PKC alpha Monoclonal Antibody (MC5)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6个已发表图片

6篇引用文献

查看其他37个PKC alpha抗体

说明书

实验方案

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说明书

实验方案

技术支持

Product Image Gallery

PKC alpha Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of PKC alpha (green) in SH-SY5Y and MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a PKC alpha monoclonal antibody (Product # MA1-157) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502). F-Actin (red) was stained with DyLight-554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

PKC alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PKC alpha showing staining in the cytoplasm of paraffin-embedded mouse kidney tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a PKC alpha monoclonal antibody (Product # MA1-157) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

PKC alpha Antibody in Western Blot (WB) — Western blot analysis of PKC alpha was performed by loading 40 µg of various whole cell lysates and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for 1 hour. The membrane was probed with a PKC alpha monoclonal antibody (Product # MA1-157) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1% Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075) (upper panel). To assay for protein loading (lower panel), the membrane was stripped with Restore PLUS Western Blot Stripping Buffer (Product # 46430), blocked with 5% BSA/TBST and re-probed with an HRP-conjugated Beta Actin Loading Control Antibody (Product # MA5-15739-HRP) at a dilution of 1:1000 for 1 hour at room temperature. The membrane was washed in TBS-0.1% Tween-20 and chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

PKC alpha Antibody in Immunoprecipitation (IP) — Immunoprecipitation of PKC alpha was performed using Jurkat whole cell lysate. Antigen-antibody complexes were formed by incubating 400 µg of lysate with 5 µg of a PKC alpha monoclonal antibody (Product # MA1-157) overnight on a rocking platform at 4°C. The immune complexes were captured on 50 µL Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Jurkat cell lysate (40 µg) was loaded as a positive control (left lane). The sample was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for 1 hour. The membrane was probed with a PKC alpha monoclonal antibody (Product # MA1-157) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

PKC alpha Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. NIH/3T3 cells were transfected with PKC alpha siRNA and loss of signal was observed in Western Blot using anti-PKC alpha Monoclonal Antibody (Product # MA1-157). {KD}

PKC alpha Antibody in Immunohistochemistry (IHC) — FIGURE 5 Rabbit retinal photoreceptors across the entire retina were damaged by photocoagulation with 100 mW laser power, 50 ms pulse duration, and 500 mum laser spot size. (A,B) Fundus photographs of extensive damage of retinal photoreceptors at 1 h and 7 days after photocoagulation. Scale bar = 2 mm. (C) Control OCT image of rabbit retina. Scale bar = 1 mm. (D) Example of OCT image of rabbit retina at 7 days after photocoagulation. Scale bar = 1 mm. Red lines in (C,D) mark retina stratification. (E,F) Histomorphology of control rabbit retina (E) and rabbit retina 7 days after photocoagulation (F) . Scale bar = 100 mum. (G,H) Immunofluorescence staining of control rabbit retina (G) and rabbit retina 7 days after photocoagulation (H) with cone outer segments marker PNA, bipolar cell marker PKC-alpha and amacrine cell marker ChAT 7 days after photocoagulation. Scale bar = 100 mum. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer.

产品信息

MA1-157

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:1,000	-
免疫组化 (IHC)	-	查看5篇已发表文章 5篇已发表文章
免疫组化（石蜡） (IHC (P))	1:10-1:100	-
免疫细胞化学 (ICC/IF)	1:50-1:100	查看1篇已发表文章 1篇已发表文章
免疫沉淀 (IP)	5 µg	-

产品规格
种属反应	Bovine, Dog, Human, Mouse, Non-human primate, Rabbit, Rat
已发表种属	Mouse, Rabbit
宿主/亚型	Mouse / IgG2a
分类	Monoclonal
类型	Antibody
克隆号	MC5
抗原	Purified bovine brain Protein Kinase C alpha.
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Protein A
保存液	PBS with 30% glycerol, 1mg/mL BSA
内含物	0.05% sodium azide
保存条件	-20°C
RRID	AB_2536865

产品详细信息

Western blot analysis of MA1-157 detects an ~80 kDa protein in human, bovine, non-human primate, mouse, rat, dog and rabbit cells. In Jurkat, rat brain and MDCK cell lysates, a weak unknown band at ~140 kDa is also detected. MA1-157 shows specificity to PKC alpha and is non-reactive to other PKC family members.

靶标信息

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Large-Area Photoreceptor Degeneration Model in Rabbits by Photocoagulation and Oxidative Stress in the Retina.

Frontiers in neuroscience

Wang Z,Feng C,Yang R,Liu T,Chen Y,Chen A,Yan B,Yuan Y,Zhang J

MA1-157 was used in Immunohistochemistry to examine different parameters of photocoagulation on lesions of the retina in a rabbit.

Thu Aug 12 00:00:00 EDT 2021

Large-Area Photoreceptor Degeneration Model in Rabbits by Photocoagulation and Oxidative Stress in the Retina.

Frontiers in neuroscience

Wang Z,Feng C,Yang R,Liu T,Chen Y,Chen A,Yan B,Yuan Y,Zhang J

MA1-157 was used in Immunohistochemistry to examine different parameters of photocoagulation on lesions of the retina in a rabbit.

Thu Aug 12 00:00:00 EDT 2021

Prion-induced photoreceptor degeneration begins with misfolded prion protein accumulation in cones at two distinct sites: cilia and ribbon synapses.

Acta neuropathologica communications

Striebel JF,Race B,Leung JM,Schwartz C,Chesebro B

MA1-157 was used in Immunocytochemistry to study deposition of disease-associated prion protein (PrPSc) and its association with damage to critical retinal structures following intracerebral prion inoculation.

Fri Jan 29 00:00:00 EST 2021

Prion-induced photoreceptor degeneration begins with misfolded prion protein accumulation in cones at two distinct sites: cilia and ribbon synapses.

Acta neuropathologica communications

Striebel JF,Race B,Leung JM,Schwartz C,Chesebro B

Fri Jan 29 00:00:00 EST 2021

Prion-induced photoreceptor degeneration begins with misfolded prion protein accumulation in cones at two distinct sites: cilia and ribbon synapses.

Acta neuropathologica communications

Striebel JF,Race B,Leung JM,Schwartz C,Chesebro B

Fri Jan 29 00:00:00 EST 2021

A dual role for Ca_v1.4 Ca²⁺ channels in the molecular and structural organization of the rod photoreceptor synapse.

eLife

Maddox JW,Randall KL,Yadav RP,Williams B,Hagen J,Derr PJ,Kerov V,Della Santina L,Baker SA,Artemyev N,Hoon M,Lee A

MA1-157 was used in Immunohistochemistry to support a model in which presynaptic Cav channels serve both as organizers of synaptic building blocks and as sources of Ca2+ ions in building the first synapse of the visual pathway and perhaps more broadly in the nervous system.

Thu Sep 17 00:00:00 EDT 2020

Tmem30a deficiency leads to retinal rod bipolar cell degeneration.

Journal of neurochemistry

Yang Y,Liu W,Sun K,Jiang L,Zhu X

Published figure using PKC alpha monoclonal antibody (Product # MA1-157) in Immunofluorescence

Fri Feb 01 00:00:00 EST 2019

Localization of Rod Bipolar Cells in the Mammalian Retina Using an Antibody Against the α1c L-type Ca(2+) Channel.

Acta histochemica et cytochemica

Huh YJ,Choi JS,Jeon CJ

MA1-157 was used in immunohistochemistry to test if the alpha1c subunit of L-type voltage-gated calcium channel colocalizes with protein kinase C alpha in the rod bipolar cells in mouse, hamster, rabbit, and dog retinas

Tue Apr 28 00:00:00 EDT 2015

生物信息学

蛋白别名: aging-associated gene 6; EC 2.7.11.13; FHC; FTH; FTHL6; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; PKC-A; PKC-alpha; PKC-III; PLIF; protein kinase C; Protein kinase C alpha type; protein kinase C, alpha; protein kinase c-alpha; unnamed protein product

基因别名: AAG6; AI875142; PKC-alpha; PKCA; PKRCA; PRKACA; PRKCA

UniProt ID: (Bovine) P04409, (Rabbit) P10102, (Human) P17252, (Mouse) P20444

Entrez Gene ID: (Bovine) 282001, (Rabbit) 100037720, (Human) 5578, (Dog) 490904, (Mouse) 18750, (Rat) 24680

分子生物学功能: non-receptor serine/threonine protein kinase