Invitrogen

PKC epsilon Polyclonal Antibody

查看其他12个PKC epsilon抗体

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PKC epsilon Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of PKC epsilon in paraffin-embedded human esophageal cancer using PKC epsilon Polyclonal Antibody (Product # PA5-96060) at a dilution of 1:100.

PKC epsilon Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of PKC epsilon in paraffin-embedded rat spleen using PKC epsilon Polyclonal Antibody (Product # PA5-96060) at a dilution of 1:200.

PKC epsilon Antibody in Western Blot (WB) — Western Blot analysis of PKC epsilon in extracts of various cell lines using PKC epsilon Polyclonal Antibody (Product # PA5-96060) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

产品信息

PA5-96060

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫组化（石蜡） (IHC (P))	1:50 - 1:200	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant fusion protein containing a sequence corresponding to amino acids 488-737 of human PRKCE.
偶联物	Unconjugated
形式	Liquid
浓度	1.11 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.3, with 50% glycerol
内含物	0.02% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2807862

产品详细信息

Immunogen sequence: YVNGGDLMFQ IQRSRKFDEP RSRFYAAEVT SALMFLHQHG VIYRDLKLDN ILLDAEGHCK LADFGMCKEG ILNGVTTTTF CGTPDYIAPE ILQELEYGPS VDWWALGVLM YEMMAGQPPF EADNEDDLFE SILHDDVLYP VWLSKEAVSI LKAFMTKNPH KRLGCVASQN GEDAIKQHPF FKEIDWVLLE QKKIKPPFKP RIKTKRDVNN FDQDFTREEP VLTLVDEAIV KQINQEEFKG FSYFGEDLMP; Positive Samples: 293T, HT-29, Mouse brain, Mouse lung; Cellular Location: Cell membrane, Cytoplasm, Nucleus, cytoskeleton, perinuclear region

靶标信息

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: MGC125656; MGC125657; nPKC-epsilon; PKC e; Protein kinase C epsilon type; protein kinase C, epsilon

基因别名: 5830406C15Rik; nPKC-epsilon; PKC[e]; PKCE; Pkcea; PKCepsilon; PRKCE; R75156

UniProt ID: (Human) Q02156, (Mouse) P16054, (Rat) P09216

Entrez Gene ID: (Human) 5581, (Mouse) 18754, (Rat) 29340

分子生物学功能: non-receptor serine/threonine protein kinase protein modifying enzyme

macrophage activation involved in immune response protein phosphorylation apoptotic process cell cycle cell adhesion signal transduction activation of phospholipase C activity positive regulation of epithelial cell migration positive regulation of fibroblast migration positive regulation of cell-substrate adhesion peptidyl-serine phosphorylation platelet activation positive regulation of actin filament polymerization negative regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion positive regulation of synaptic transmission, GABAergic positive regulation of cytokinesis intracellular signal transduction locomotory exploration behavior TRAM-dependent toll-like receptor 4 signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of I-kappaB kinase/NF-kappaB signaling response to morphine positive regulation of MAPK cascade regulation of peptidyl-tyrosine phosphorylation positive regulation of lipid catabolic process release of sequestered calcium ion into cytosol regulation of release of sequestered calcium ion into cytosol cell division regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of mucus secretion cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of wound healing positive regulation of receptor activity negative regulation of sodium ion transmembrane transporter activity positive regulation of cellular glucuronidation immune system process chemosensory behavior negative regulation of mitochondrial membrane potential phosphorylation regulation of lipid metabolic process cellular response to platelet-derived growth factor stimulus negative regulation of apoptotic process positive regulation of catalytic activity negative regulation of release of sequestered calcium ion into cytosol negative regulation of mitochondrial calcium ion concentration

参与通路

actin monomer binding protein serine/threonine kinase activity protein kinase C activity calcium-independent protein kinase C activity signal transducer activity protein binding ATP binding enzyme activator activity enzyme binding receptor activator activity ethanol binding metal ion binding 14-3-3 protein binding nucleotide binding protein kinase activity receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups SH3 domain binding protein kinase binding