Invitrogen

PKC eta Recombinant Rabbit Monoclonal Antibody (JE32-41)

PKC eta Antibody in Immunocytochemistry (ICC/IF)

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PKC eta Antibody (MA562411) in ICC/IF

Immunofluorescence analysis of PKC eta/PRKCH was performed using 70% confluent log phase BeWo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PKC eta Recombinant Rabbit Monoclonal Antibody (JE32-41) (Product # MA562411) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2,000 dilutio... View More

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产品信息

MA562411

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE32-41

抗原

Recombinant protein within Mouse PKC eta aa 584-683/683.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive Control: MCF7 cell lysate, A431 cell lysate, COS-1 cell lysate, mouse brain tissue lysate, rat brain tissue lysate.

Tissue Specificity: Low tissue specificity.

Subcellular Location: Cytoplasm.

Sequence Similarities: 97% Mouse/Rat.

Predicted band size: 78 kDa.

靶标信息

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。