Invitrogen

PKM2 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1个已发表图片

3 篇参考文献

查看其他31个PKM2抗体

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说明书

实验方案

技术支持

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PKM2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PKM2 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PKM2 Polyclonal Antibody (Product # PA5-23034) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PKM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PKM2 in mouse liver tissue. Samples were incubated in PKM2 polyclonal antibody (Product # PA5-23034).

PKM2 Antibody in Western Blot (WB) — Knockdown of PKM2 was achieved by transfecting MDA-MB-231 cells with PKM2 specific siRNAs (Silencer® select Product # s10574). Western blot analysis (Fig. a) was performed using whole cell extracts from the PKM2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with PKM2 Polyclonal Antibody (Product # PA5-23034, 0.5 µg/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PKM2.

PKM2 Antibody in Flow Cytometry (Flow) — Flow cytometry of PKM2 in HeLa cells (blue) and a matched isotype control (orange). Samples were incubated in PKM2 polyclonal antibody (Product # PA5-23034) using a dilution of 5 µg/mL for 30 minutes at room temperature. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 488.

PKM2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MDAMB231 cells were transfected with PKM2 siRNA and reduction of signal was observed in Western Blot using PKM2 Polyclonal Antibody (Product # PA5-23034). {KD}

PKM2 Antibody in Immunohistochemistry (IHC) — Figure 3 Reporter gene expression is stable and restricted to hepatocytes after pBDL following neonatal intravascular administration of lentiviral vectors. SFFV-FLuc/eGFP lentivirus was administered to P1 neonatal mice by intravascular (i.v.) injection and then subjected to pBDL or sham pBDL in adulthood. ( A ) Mice were subjected to continued luciferase bioimaging over 40 days where no change in luciferase activity was observed over time or between pBDL and sham groups (n = 10 pBDL, 5 sham, not significant, Student's t-test). Mice were sacrificed 90 days after pBDL and co-immunostained for GFP and markers of ( Bi-iii ) hepatocytes; HNF4alpha, ( C-iii ) biliary epithelia; CK7, ( Di-iii ) hepatic progenitors; PKM2, ( Ei-iii ) myofibroblasts; alphaSMA and ( Fi-iii ) hepatic stellate cells; GFAP (all groups n = 3-6).

产品信息

PA5-23034

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.5 µg/mL	查看2篇已发表文章 2篇已发表文章
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	1:100	-
免疫细胞化学 (ICC/IF)	1:200	-
流式细胞分析 (Flow)	5 µg/mL	-
免疫沉淀 (IP)	Assay-Dependent	-

产品规格
种属反应	Bovine, Human, Mouse, Rat
已发表种属	Human, Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	A synthetic peptide made to the internal region of human PKM2 protein (within residues 350-450).
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS with 30% glycerol
内含物	0.1% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_11151984

产品详细信息

The target sequence has 95% sequence homology with bovine.

靶标信息

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Longitudinal in vivo bioimaging of hepatocyte transcription factor activity following cholestatic liver injury in mice.

Scientific reports

Delhove JM,Buckley SM,Perocheau DP,Karda R,Arbuthnot P,Henderson NC,Waddington SN,McKay TR

PA523034 was used in immunohistochemistry to report that hepatocytes rapidly upregulate NF- kB and Smad2/3 activity, and repress Notch signaling

Fri Feb 03 00:00:00 EST 2017

Breast cancer stem cells rely on fermentative glycolysis and are sensitive to 2-deoxyglucose treatment.

Cell death & disease

Ciavardelli D,Rossi C,Barcaroli D,Volpe S,Consalvo A,Zucchelli M,De Cola A,Scavo E,Carollo R,D'Agostino D,Forlì F,D'Aguanno S,Todaro M,Stassi G,Di Ilio C,De Laurenzi V,Urbani A

PA5-23034 was used in western blot to identify the metabolic phenotype associated with breast cancer stem cells

Thu Jul 17 00:00:00 EDT 2014

p63 isoforms regulate metabolism of cancer stem cells.

Journal of proteome research

D'Aguanno S,Barcaroli D,Rossi C,Zucchelli M,Ciavardelli D,Cortese C,De Cola A,Volpe S,D'Agostino D,Todaro M,Stassi G,Di Ilio C,Urbani A,De Laurenzi V

PA5-23034 was used in western blot to study the modulation of colon cancer stem cell metabolism by p63

Fri Apr 04 00:00:00 EDT 2014

生物信息学

蛋白别名: CTHBP; Cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; isozymes M1/M2; m2pk; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; Pyruvate kinase 2/3; pyruvate kinase 3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; pyruvate kinase, muscle; THBP1; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tu; Tumor M2-PK

基因别名: AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; Pk-2; Pk-3; PK2; PK3; PKM; PKM12; PKM2; Pykm; TCB; THBP1

UniProt ID: (Human) B4DUU6, (Mouse) P52480, (Rat) P11980

Entrez Gene ID: (Bovine) 512571, (Human) 5315, (Mouse) 18746, (Rat) 25630

分子生物学功能: kinase

功能

response to hypoxia liver development ATP biosynthetic process response to nutrient response to gravity programmed cell death response to muscle inactivity organ regeneration response to insulin pyruvate biosynthetic process skeletal muscle tissue regeneration canonical glycolysis cell-cell adhesion glucose metabolic process glycolytic process metabolic process phosphorylation response to organic substance

参与通路

magnesium ion binding pyruvate kinase activity protein binding ATP binding kinase activity MHC class II protein complex binding potassium ion binding ADP binding poly(A) RNA binding cadherin binding involved in cell-cell adhesion nucleotide binding catalytic activity transferase activity metal ion binding

PKM2 Polyclonal Antibody

产品信息

PA5-23034

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

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生物信息学

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