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OriGene
PLEK Monoclonal Antibody (OTI4F10), TrueMAB™
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PLEK Antibody (CF500899) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLEK (RC203780, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PLEK. Positive lysates LY419171 (100 µg) and LC419171 (20 µg) can be purchased separately from OriGene.
产品信息
CF500899
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
OTI4F10
抗原
Full length human recombinant protein of human PLEK produced in HEK293T cell.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 8% trehalose
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
靶标信息
Major protein kinase C substrate of platelets.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ27168; p47; Platelet 47 kDa protein; Pleckstrin
基因别名: P47; PLEK
UniProt ID: (Human) P08567
Entrez Gene ID: (Human) 5341
hematopoietic progenitor cell differentiation
platelet degranulation
vesicle docking involved in exocytosis
integrin-mediated signaling pathway
positive regulation of platelet activation
negative regulation of inositol phosphate biosynthetic process
positive regulation of inositol-polyphosphate 5-phosphatase activity
cell projection organization
positive regulation of actin filament depolymerization
phospholipase C-inhibiting G-protein coupled receptor signaling pathway
cortical actin cytoskeleton organization
ruffle organization
actin cytoskeleton reorganization
positive regulation of actin filament bundle assembly
positive regulation of integrin activation
negative regulation of G-protein coupled receptor protein signaling pathway
phosphatidylinositol metabolic process
negative regulation of calcium-mediated signaling
regulation of cell diameter
thrombin receptor signaling pathway
platelet aggregation
protein kinase C signaling
protein secretion by platelet
