Invitrogen

PLK1 Monoclonal Antibody (35-206)

PLK1 Antibody in Immunocytochemistry (ICC/IF)

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PLK1 Antibody (37-7000) in ICC/IF

Immunofluorescent staining of (top row) U2-OS cells, (bottom row) HeLa S3 cells using Ms anti PLK-1 (Product # 37-7000). Image courtesy of Dr. Erich Nigg, Max-Planck Institut fur Biochemie, Germany.

PLK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PLK1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PLK1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PLK1 siRNA and decrease in signal intensity was observed in western blot application (Fig a) using PLK1 Mouse Monoclonal Antibody (Product # 37-7000). Densitometric analysis of this western blot is shown in histogram (Fig b). {KD}

产品信息

37-7000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

Assay-dependent

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流式细胞分析 (Flow)

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酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

35-206

抗原

Full-length human Plk1 (polo-like kinase 1)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533335

靶标信息

PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang KH,Zou YJ,Shan MM,Pan ZN,Ju JQ,Liu JC,Ji YM,Sun SC

Published figure using PLK1 monoclonal antibody (Product # 37-7000) in Western Blot

Mon Jan 01 00:00:00 UTC 2024

Integrin-Mediated Adhesion Promotes Centrosome Separation in Early Mitosis.

Cells

Kamranvar SA,Gupta DK,Wasberg A,Liu L,Roig J,Johansson S

Published figure using PLK1 monoclonal antibody (Product # 37-7000) in Western Blot

Sat Apr 16 00:00:00 UTC 2022

A novel association of pyroptosis-related gene signature with the prognosis of hepatocellular carcinoma.

Frontiers in oncology

Li Y,Li Y,Zhang X,Duan X,Feng H,Yu Z,Gao Y

37-7000 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that the three-PRG signature act as independent factor to predict the prognosis of HCC patients.

Sat Oct 22 00:00:00 UTC 2022

Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines.

STAR protocols

Xie G,Zhou Y,Du M,Wang Q,Yi J,Zhang XK

37-7000 was used in Immunocytochemistry to develop a new protocol demonstrating nuclear receptor RXRa centrosomal translocation which may be applied to other transcription factors.

Fri Sep 17 00:00:00 UTC 2021

Methylation of PLK1 by SET7/9 ensures accurate kinetochore-microtubule dynamics.

Journal of molecular cell biology

Yu R,Wu H,Ismail H,Du S,Cao J,Wang J,Ward T,Yang F,Gui P,Ali M,Chu L,Mo F,Wang Q,Chu Y,Zang J,Zhao Y,Ye M,Fang G,Chen PR,Dou Z,Gao X,Wang W,Liu X,Yao X

Published figure using PLK1 monoclonal antibody (Product # 37-7000) in Immunoprecipitation

Fri Jul 03 00:00:00 UTC 2020

Methylation of PLK1 by SET7/9 ensures accurate kinetochore-microtubule dynamics.

Journal of molecular cell biology

Yu R,Wu H,Ismail H,Du S,Cao J,Wang J,Ward T,Yang F,Gui P,Ali M,Chu L,Mo F,Wang Q,Chu Y,Zang J,Zhao Y,Ye M,Fang G,Chen PR,Dou Z,Gao X,Wang W,Liu X,Yao X

Published figure using PLK1 monoclonal antibody (Product # 37-7000) in Immunoprecipitation

Fri Jul 03 00:00:00 UTC 2020

Temporal and SUMO-specific SUMOylation contribute to the dynamics of Polo-like kinase 1 (PLK1) and spindle integrity during mouse oocyte meiosis.

Developmental biology

Feitosa WB,Hwang K,Morris PL

37-7000 was used in Immunocytochemistry-immunoflourescence to study how both temporal and Small ubiquitin-related modifier-specific-SUMOylation play important roles in orchestrating functional dynamics of Polo-like kinase 1 during mouse oocyte meiosis, including subcellular compartmentalisation.

Thu Feb 15 00:00:00 UTC 2018

YPC-21661 and YPC-22026, novel small molecules, inhibit ZNF143 activity in vitro and in vivo.

Cancer science

Haibara H,Yamazaki R,Nishiyama Y,Ono M,Kobayashi T,Hokkyo-Itagaki A,Nishisaka F,Nishiyama H,Kurita A,Matsuzaki T,Izumi H,Kohno K

37-7000 was used in Western Blotting to show that the inhibition of Zinc-finger protein 143 activity by small molecules induces tumour regression in vitro and in vivo.

Mon May 01 00:00:00 UTC 2017

A novel form of necrosis, TRIAD, occurs in human Huntington's disease.

Acta neuropathologica communications

Yamanishi E,Hasegawa K,Fujita K,Ichinose S,Yagishita S,Murata M,Tagawa K,Akashi T,Eishi Y,Okazawa H

377000 was used in western blot to assess structural and biochemical phenotypes of neuronal transcriptional repression-induced atypical cell death in patient brains with Huntington's disease

Wed Mar 08 00:00:00 UTC 2017

A novel form of necrosis, TRIAD, occurs in human Huntington's disease.

Acta neuropathologica communications

Yamanishi E,Hasegawa K,Fujita K,Ichinose S,Yagishita S,Murata M,Tagawa K,Akashi T,Eishi Y,Okazawa H

377000 was used in western blot to assess structural and biochemical phenotypes of neuronal transcriptional repression-induced atypical cell death in patient brains with Huntington's disease

Wed Mar 08 00:00:00 UTC 2017

Hypoxia-induced alterations of G2 checkpoint regulators.

Molecular oncology

Hasvold G,Lund-Andersen C,Lando M,Patzke S,Hauge S,Suo Z,Lyng H,Syljuåsen RG

37-7000 was used in flow cytometry to analyze G2 checkpoint regulators for hypoxia-induced alterations

Sun May 01 00:00:00 UTC 2016

Monitoring of changes in lipid profiles during PLK1 knockdown in cancer cells using DESI MS.

Analytical and bioanalytical chemistry

Jayashree B,Srimany A,Jayaraman S,Bhutra A,Janakiraman N,Chitipothu S,Krishnakumar S,Baddireddi LS,Elchuri S,Pradeep T

37-7000 was used in western blot to utilize DESI MS for PLK1 knockdown in cancer cells to monitor changes in lipid profiles

Mon Aug 01 00:00:00 UTC 2016

The hnRNP-Htt axis regulates necrotic cell death induced by transcriptional repression through impaired RNA splicing.

Cell death & disease

Mao Y,Tamura T,Yuki Y,Abe D,Tamada Y,Imoto S,Tanaka H,Homma H,Tagawa K,Miyano S,Okazawa H

Published figure using PLK1 monoclonal antibody (Product # 37-7000) in Western Blot

Thu Apr 28 00:00:00 UTC 2016

Targeting TEAD/YAP-transcription-dependent necrosis, TRIAD, ameliorates Huntington's disease pathology.

Human molecular genetics

Mao Y,Chen X,Xu M,Fujita K,Motoki K,Sasabe T,Homma H,Murata M,Tagawa K,Tamura T,Kaye J,Finkbeiner S,Blandino G,Sudol M,Okazawa H

37-7000 was used in Western Blot to suggest approaches targeting the signalling pathway of TEAD/YAP-transcription-dependent necrosis (TRIAD) could lead to a therapeutic development against HD.

Tue Nov 01 00:00:00 UTC 2016

Aberrant expression of maternal Plk1 and Dctn3 results in the developmental failure of human in-vivo- and in-vitro-matured oocytes.

Scientific reports

Fan Y,Zhao HC,Liu J,Tan T,Ding T,Li R,Zhao Y,Yan J,Sun X,Yu Y,Qiao J

37-7000 was used in western blot to compare gene expression profiles of oocytes and zygotes and focus on cytokinesis-related genes

Tue Feb 03 00:00:00 UTC 2015

Aberrant expression of maternal Plk1 and Dctn3 results in the developmental failure of human in-vivo- and in-vitro-matured oocytes.

Scientific reports

Fan Y,Zhao HC,Liu J,Tan T,Ding T,Li R,Zhao Y,Yan J,Sun X,Yu Y,Qiao J

37-7000 was used in western blot to compare gene expression profiles of oocytes and zygotes and focus on cytokinesis-related genes

Tue Feb 03 00:00:00 UTC 2015

Wee1 is required to sustain ATR/Chk1 signaling upon replicative stress.

Oncotarget

Saini P,Li Y,Dobbelstein M

37-7000 was used in western blot to compare the chemosensitizing effect of nucleoside analogues in cells derived from pancreatic cancer and in osteosarcoma-derived cells

Sat May 30 00:00:00 UTC 2015

SAPK pathways and p53 cooperatively regulate PLK4 activity and centrosome integrity under stress.

Nature communications

Nakamura T,Saito H,Takekawa M

37-7000 was used in western blot to study the regulation of polo-like kinase 4 activity and centrosome numeral integrity by SAPK pathways and p53.

Tue Nov 26 00:00:00 UTC 2013

Proteomic profiling revealed the functional networks associated with mitotic catastrophe of HepG2 hepatoma cells induced by 6-bromine-5-hydroxy-4-methoxybenzaldehyde.

Toxicology and applied pharmacology

Zhang B,Huang B,Guan H,Zhang SM,Xu QZ,He XP,Liu XD,Wang Y,Shang ZF,Zhou PK

Sun May 01 00:00:00 UTC 2011

Proteomic profiling revealed the functional networks associated with mitotic catastrophe of HepG2 hepatoma cells induced by 6-bromine-5-hydroxy-4-methoxybenzaldehyde.

Toxicology and applied pharmacology

Zhang B,Huang B,Guan H,Zhang SM,Xu QZ,He XP,Liu XD,Wang Y,Shang ZF,Zhou PK

Sun May 01 00:00:00 UTC 2011

Three independent mechanisms for arrest in G2 after ionizing radiation.

Cell cycle (Georgetown, Tex.)

Landsverk KS,Patzke S,Rein ID,Stokke C,Lyng H,De Angelis PM,Stokke T

37-7000 was used in western blot to identify and characterize radiation-induced G2-arrest in human B-lymphocyte cancer cell lines.

Tue Mar 01 00:00:00 UTC 2011

Forced Expression of ZNF143 Restrains Cancer Cell Growth.

Cancers

Izumi H,Yasuniwa Y,Akiyama M,Yamaguchi T,Kuma A,Kitamura N,Kohno K

37-7000 was used in Western Blotting to assess the function of ZNF143 expression in the cell cycle and cell division.

Wed Oct 19 00:00:00 UTC 2011

Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis.

Science signaling

Wang Z,Udeshi ND,Slawson C,Compton PD,Sakabe K,Cheung WD,Shabanowitz J,Hunt DF,Hart GW

Tue Jan 12 00:00:00 UTC 2010

Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis.

Science signaling

Wang Z,Udeshi ND,Slawson C,Compton PD,Sakabe K,Cheung WD,Shabanowitz J,Hunt DF,Hart GW

Tue Jan 12 00:00:00 UTC 2010

Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis.

Science signaling

Wang Z,Udeshi ND,Slawson C,Compton PD,Sakabe K,Cheung WD,Shabanowitz J,Hunt DF,Hart GW

Tue Jan 12 00:00:00 UTC 2010

Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis.

Developmental cell

Fabbro M,Zhou BB,Takahashi M,Sarcevic B,Lal P,Graham ME,Gabrielli BG,Robinson PJ,Nigg EA,Ono Y,Khanna KK

37-7000 was used in immunocytochemistry, immunoprecipitation, and western blot to elucidate how Cep55 regulates cytokinesis

Sat Oct 01 00:00:00 UTC 2005

生物信息学

蛋白别名: cell cycle regulated protein kinase; PLK 1; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14

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基因别名: PLK; PLK1; STPK13

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UniProt ID: (Human) P53350, (Mouse) Q07832, (Rat) Q62673

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Entrez Gene ID: (Human) 5347, (Mouse) 18817, (Rat) 25515

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功能