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Invitrogen
PMEL Polyclonal Antibody
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PMEL Antibody (PA5-32491) in WB
Western blot was performed using Anti-PMEL Polyclonal Antibody (Product # PA5-32491) and an 80 kDa band corresponding to PMEL was observed across in SK-MEL-5 in comparison to A-375 and SK-BR-3 which is supposed to be negative. A non-specific band around 58 kDa was also observed. Whole cell extracts (30 µg lysate) of SK-MEL-5 (Lane 1), A-375 (Lane 2) and SK-BR-3 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB230... View More
产品信息
PA5-32491
产品规格
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encoding aa 1-150 human gp100.
偶联物
形式
Liquid
浓度
0.123 mg/mL
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is melanoma.
靶标信息
Melanoma is a malignant tumor of melanocytes which are found predominantly in skin but also in the bowel and the eye (see uveal melanoma). It is one of the rarer types of skin cancer but causes the majority of skin cancer related deaths.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
