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Invitrogen
PNP Polyclonal Antibody
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PNP Antibody (PA5-80914) in WB
Western Blot using PNP Polyclonal Antibody (Product # PA5-80914) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: K562 Whole Cell Lysate, Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa.
产品信息
PA5-80914
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human PNP protein (Met1-Ser289)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human PNP.
靶标信息
The PNP gene encodes for the enzyme purine nucleoside phosphorylase, which plays a vital role in the purine salvage pathway by catalyzing the reversible cleavage of purine nucleosides. This enzyme facilitates the conversion of inosine, guanosine, and deoxyguanosine into free bases and sugars phosphorylated at the C1 position. PNP is located on chromosome 14q13 and is crucial for nucleoside catabolism, impacting energy metabolism and cellular functions across various organisms. PNP deficiency is an autosomal recessive multisystem disorder arising from mutations in the PNP gene, leading to impaired T-cell maturation and function, resulting in recurrent infections, autoimmune manifestations, and neurological abnormalities due to the toxic accumulation of deoxyguanosine triphosphate (dGTP). This deficiency particularly affects immature thymocytes and can lead to lymphoid and neuronal toxicity, underscoring the gene's importance in proper immune system function and cellular health.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: epididymis secretory sperm binding protein Li 156an; HEL-S-156an; Inosine phosphorylase; Inosine-guanosine phosphorylase; PNP; Purine nucleoside phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase
基因别名: NP; PNP; PRO1837; PUNP
UniProt ID: (Human) P00491
Entrez Gene ID: (Human) 4860
nucleobase-containing compound metabolic process
inosine catabolic process
purine nucleotide catabolic process
nicotinamide riboside catabolic process
immune response
NAD biosynthesis via nicotinamide riboside salvage pathway
urate biosynthetic process
positive regulation of T cell proliferation
response to drug
purine-containing compound salvage
positive regulation of alpha-beta T cell differentiation
interleukin-2 secretion
