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Invitrogen
PORCN Polyclonal Antibody
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PORCN Antibody (PA5-88445) in WB
Western blot analysis of PORCN in extracts of various cell lines. Samples were incubated with PORCN Polyclonal antibody (Product # PA5-88445) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
产品信息
PA5-88445
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 220-320 of human PORCN (NP_9822991)
偶联物
形式
Liquid
浓度
1.46 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: PLNGDRLLRN KKRKARWLRA YESAVSFHFS NYFVGFLSEA TATLAGAGFT EEKDHLEWDL TVSKPLNVEL PRSMVEVVTS WNLPMSYWLN NYVFKNALRL G; Positive Samples: Mouse kidney, Mouse liver, Rat kidney; Cellular Location: Endoplasmic reticulum membrane, Multi-pass membrane protein
靶标信息
PORCN belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the Porc family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.This gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the Porc family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: MGC29687; mPORC; por; protein-cysteine N-palmitoyltransferase porcupine; Protein-serine O-palmitoleoyltransferase porcupine
基因别名: 2410004O13Rik; AW045557; DXHXS7465e; Mg61; mMg61; Mporc; Porc; Porcn; Ppn; RGD1564947
UniProt ID: (Mouse) Q9JJJ7
Entrez Gene ID: (Mouse) 53627, (Rat) 317368
