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Invitrogen
POU5F2 Polyclonal Antibody
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POU5F2 Antibody (PA5-103510) in ICC/IF
Immunocytochemistry analysis of POU5F2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with POU5F2 polyclonal antibody (Product # PA5-103510) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-103510
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human POU5F2(Accession Q8N7G0), corresponding to amino acid residues H242-F292.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total POU5F2.
靶标信息
Transcription factor that binds preferentially to the octamer motif (5'-ATGTTAAT-3'). May exert a regulatory function in meiotic events that are required for terminal differentiation of male germ cell.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: POU domain, class 5, transcription factor 2; Sperm 1 POU domain transcription factor; sperm-associated POU domain protein 1; SPRM-1
基因别名: 1700013G10Rik; POU5F2; SPRM-1; SPRM1
UniProt ID: (Human) Q8N7G0, (Mouse) Q9DAC9
Entrez Gene ID: (Human) 134187, (Mouse) 75507
