Invitrogen

PPAR gamma Monoclonal Antibody (K.242.9)

PPAR gamma Antibody in Immunocytochemistry (ICC/IF)

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PPAR gamma Antibody (MA5-14889) in ICC/IF

Immunofluorescent analysis of PPAR-gamma in 3T3-L1 cells using a PPAR-gamma monoclonal antibody (Product # MA5-14889) (red) showing nuclear localization in differentiated cells. Lipid droplets have been labeled with a BODIPY fluorescent dye (green). DNA is labeled using a fluorescent blue dye.

PPAR gamma Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PPAR gamma Antibody in Western Blot (WB)

PPAR gamma Antibody in Immunohistochemistry (IHC)

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产品信息

MA5-14889

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看13篇已发表文章 13篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:400-1:1,600

免疫细胞化学 (ICC/IF)

1:50-1:200

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

K.242.9

抗原

Synthetic peptide corresponding to residues surrounding Asp69 of human PPAR-gamma

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

125 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10985650

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Deletion of Spinophilin Promotes White Adipocyte Browning.

Pharmaceuticals (Basel, Switzerland)

Gou W,Wei H,Swaby L,Green E,Wang H

MA5-14889 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate the critical role of SPL in the regulation of WAT browning.

Sun Jan 08 00:00:00 UTC 2023

Deletion of Spinophilin Promotes White Adipocyte Browning.

Pharmaceuticals (Basel, Switzerland)

Gou W,Wei H,Swaby L,Green E,Wang H

MA5-14889 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate the critical role of SPL in the regulation of WAT browning.

Sun Jan 08 00:00:00 UTC 2023

Hepatic PPARα Is Destabilized by SIRT1 Deacetylase in Undernourished Male Mice.

Frontiers in nutrition

Suh JH,Kim KH,Conner ME,Moore DD,Preidis GA

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Western Blot

Fri Apr 29 00:00:00 UTC 2022

Hepatic PPARα Is Destabilized by SIRT1 Deacetylase in Undernourished Male Mice.

Frontiers in nutrition

Suh JH,Kim KH,Conner ME,Moore DD,Preidis GA

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Western Blot

Fri Apr 29 00:00:00 UTC 2022

Cell fate determining molecular switches and signaling pathways in Pax7-expressing somitic mesoderm.

Cell discovery

Fung CW,Zhou S,Zhu H,Wei X,Wu Z,Wu AR

MA5-14889 was used in Immunocytochemistry-immunoflourescence to provide further understanding of the Pax7 lineage during embryonic development using a combination of lineage tracing with temporally sampled single-cell transcriptomics.

Tue Jun 28 00:00:00 UTC 2022

β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight.

Biomedicines

Hidalgo-Gutiérrez A,Barriocanal-Casado E,Díaz-Casado ME,González-García P,Zenezini Chiozzi R,Acuña-Castroviejo D,López LC

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Western Blot

Wed Oct 13 00:00:00 UTC 2021

Defining the lineage of thermogenic perivascular adipose tissue.

Nature metabolism

Angueira AR,Sakers AP,Holman CD,Cheng L,Arbocco MN,Shamsi F,Lynes MD,Shrestha R,Okada C,Batmanov K,Susztak K,Tseng YH,Liaw L,Seale P

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Immunohistochemistry

Thu Apr 01 00:00:00 UTC 2021

Dermal Adipocyte Lipolysis and Myofibroblast Conversion Are Required for Efficient Skin Repair.

Cell stem cell

Shook BA,Wasko RR,Mano O,Rutenberg-Schoenberg M,Rudolph MC,Zirak B,Rivera-Gonzalez GC,López-Giráldez F,Zarini S,Rezza A,Clark DA,Rendl M,Rosenblum MD,Gerstein MB,Horsley V

MA5-14889 was used in Immunohistochemistry to identify an essential role for adipocyte lipolysis in regulating inflammation and repair after injury in skin.

Thu Jun 04 00:00:00 UTC 2020

Protective Effects of Foam Rolling against Inflammation and Notexin Induced Muscle Damage in Rats.

International journal of medical sciences

Pablos A,Ceca D,Jorda A,Rivera P,Colmena C,Elvira L,Martínez-Arnau FM,Valles SL

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Western Blot

Mon Oct 05 00:00:00 UTC 2020

Protective Effects of Foam Rolling against Inflammation and Notexin Induced Muscle Damage in Rats.

International journal of medical sciences

Pablos A,Ceca D,Jorda A,Rivera P,Colmena C,Elvira L,Martínez-Arnau FM,Valles SL

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Western Blot

Mon Oct 05 00:00:00 UTC 2020

1α, 25-dihydroxy Vitamin D3 containing fractions of Catharanthus roseus leaf aqueous extract inhibit preadipocyte differentiation and induce lipolysis in 3T3-L1 cells.

BMC complementary and alternative medicine

Borah AK,Singh A,Yasmin R,Doley R,Mattaparthi VSK,Saha S

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Western Blot

Fri Nov 29 00:00:00 UTC 2019

Cross-talk between microtubules and the linker of nucleoskeleton complex plays a critical role in the adipogenesis of human adipose-derived stem cells.

Stem cell research & therapy

Yang Y,Qu R,Fan T,Zhu X,Feng Y,Yang Y,Deng T,Peng Y,Huang W,Ouyang J,Dai J

MA5-14889 was used in Western Blotting to determine the tole of mechanical force in the determination of lineage fate in adipose-derived stem cells.

Wed May 02 00:00:00 UTC 2018

A new hope for obesity management: Boron inhibits adipogenesis in progenitor cells through the Wnt/β-catenin pathway.

Metabolism: clinical and experimental

Doğan A,Demirci S,Apdik H,Bayrak OF,Gulluoglu S,Tuysuz EC,Gusev O,Rizvanov AA,Nikerel E,Şahin F

MA514889 was used in immunocytochemistry and western blot to investigate the effects of boron treatment on adipogenesis

Sat Apr 01 00:00:00 UTC 2017

Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning.

Diabetes

Patil M,Sharma BK,Elattar S,Chang J,Kapil S,Yuan J,Satyanarayana A

MA514889 was used in western blot to study the contribution of Id1 to brown adipose tissue thermogenesis and to the regulation of beige adipocytes in white adipose tissue

Thu Jun 01 00:00:00 UTC 2017

Cytoglobin: a potential marker for adipogenic differentiation in preadipocytes in vitro.

Cytotechnology

Doğan A,Demirci S,Kıratlı B,Şahin F

MA514889 was used in immunocytochemistry and western blot to study the role of cytoglobin in adipogenesis.

Wed Feb 01 00:00:00 UTC 2017

FoxO1 antagonist suppresses autophagy and lipid droplet growth in adipocytes.

Cell cycle (Georgetown, Tex.)

Liu L,Zheng LD,Zou P,Brooke J,Smith C,Long YC,Almeida FA,Liu D,Cheng Z

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Western Blot

Tue Aug 02 00:00:00 UTC 2016

DNA Topoisomerase IIα contributes to the early steps of adipogenesis in 3T3-L1 cells.

Cellular signalling

Jacobsen RG,Mazloumi Gavgani F,Mellgren G,Lewis AE

MA514889 was used in western blot to examine the role of topoisomerase II in mitotic clonal expansion and adipogenesis

Sat Oct 01 00:00:00 UTC 2016

Tamoxifen reduces fat mass by boosting reactive oxygen species.

Cell death & disease

Liu L,Zou P,Zheng L,Linarelli LE,Amarell S,Passaro A,Liu D,Cheng Z

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Western Blot

Thu Jan 08 00:00:00 UTC 2015

Ebf2 is a selective marker of brown and beige adipogenic precursor cells.

Proceedings of the National Academy of Sciences of the United States of America

Wang W,Kissig M,Rajakumari S,Huang L,Lim HW,Won KJ,Seale P

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Immunofluorescence

Tue Oct 07 00:00:00 UTC 2014

Targeting FoxO1 with AS1842856 suppresses adipogenesis.

Cell cycle (Georgetown, Tex.)

Zou P,Liu L,Zheng L,Liu L,Stoneman RE,Cho A,Emery A,Gilbert ER,Cheng Z

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Western Blot

Thu Sep 10 00:00:00 UTC 2015

生物信息学

蛋白别名: HUMPPARG; Nuclear receptor subfamily 1 group C member 3; OTTHUMP00000185032; OTTHUMP00000185036; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator-activated nuclear receptor gamma variant 1; Peroxisome proliferator-activated receptor gamma; PPAR gamma; PPAR-gamma; PPARy

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基因别名: CIMT1; GLM1; NR1C3; PPAR-gamma; PPAR-gamma2; PPARG; PPARG1; PPARG2; PPARgamma; PPARgamma2

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UniProt ID: (Human) P37231, (Mouse) P37238

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Entrez Gene ID: (Human) 5468, (Mouse) 19016

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功能