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PPP4C Antibody (PA5-96059) in WB
Western blot analysis of PPP4C in extracts of various cell lines. Samples were incubated with PPP4C Polyclonal antibody (Product # PA5-96059) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-96059
Applications
Tested Dilution
Publications
产品规格
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C.
Conjugate
Unconjugated
形式
Liquid
浓度
0.49 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2807861
Product Specific Information
Immunogen sequence: MAEISDLDRQ IEQLRRCELI KESEVKALCA KAREILVEES NVQRVDSPVT VCGDIHGQFY DLKELFRVGG DVPETNYLFM GDFVDRGFYS VETFLLLLAL KVRYPDRITL IRGNHESRQI TQVYGFYDEC LRKYGSVTVW RYCTEIFDYL SLSAIIDGKI FCVHGGLSPS IQTLDQIRTI DRKQEVPHDG PMCDLLWSDP EDTTGWGVSP RGAGYLFGSD VVAQFNAAND IDMICRAHQL VMEGYKWHFN ETVLTVWSAP NYCYRCGNVA AILELDEHLQ KDFIIFEAAP QETRGIPSKK PVADYFL; Positive Samples: 293T, NIH/3T3, MCF-7, HO-8910PM, Mouse testis, Mouse spleen; Cellular Location: Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Target Information
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: P PPX; PP-X; pp4; PP4C; protein phosphatase 4 (formerly X), catalytic subunit; protein phosphatase 4, catalytic subunit; Protein phosphatase X; protein phosphatase X, catalytic subunit; Serine/threonine-protein phosphatase 4 catalytic subunit
Gene Aliases: 1110002D08Rik; AU016079; PP-X; PP4; PP4C; PPH3; PPP4; PPP4C; PPX
UniProt ID: (Human) P60510, (Rat) Q5BJ92, (Mouse) P97470
Entrez Gene ID: (Human) 5531, (Rat) 171366, (Mouse) 56420
Molecular Function:
protein phosphatase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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