Invitrogen

PPP4C Polyclonal Antibody

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PPP4C Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of PPP4C was performed in MCF-7 cells using PPP4C Polyclonal Antibody (Product # PA5-96059). Blue: DAPI for nuclear staining.

PPP4C Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of PPP4C in paraffin-embedded human lung cancer using PPP4C Polyclonal Antibody (Product # PA5-96059) at a dilution of 1:100.

PPP4C Antibody in Western Blot (WB) — Western Blot analysis of PPP4C in extracts of various cell lines using PPP4C Polyclonal Antibody (Product # PA5-96059) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

PPP4C Antibody in Immunoprecipitation (IP) — Immunoprecipitation analysis of PPP4C was performed in 200 µg extracts of 293T cells using PPP4C Polyclonal Antibody (Product # PA5-96059). Western blot was performed from the immunoprecipitate using PPP4C Polyclonal Antibody at a dilution of 1:1000.

产品信息

PA5-96059

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫组化（石蜡） (IHC (P))	1:50 - 1:200	-
免疫细胞化学 (ICC/IF)	1:50 - 1:100	-
免疫沉淀 (IP)	1:50 - 1:200	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C.
偶联物	Unconjugated
形式	Liquid
浓度	1.16 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.3, with 50% glycerol
内含物	0.02% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2807861

产品详细信息

Immunogen sequence: MAEISDLDRQ IEQLRRCELI KESEVKALCA KAREILVEES NVQRVDSPVT VCGDIHGQFY DLKELFRVGG DVPETNYLFM GDFVDRGFYS VETFLLLLAL KVRYPDRITL IRGNHESRQI TQVYGFYDEC LRKYGSVTVW RYCTEIFDYL SLSAIIDGKI FCVHGGLSPS IQTLDQIRTI DRKQEVPHDG PMCDLLWSDP EDTTGWGVSP RGAGYLFGSD VVAQFNAAND IDMICRAHQL VMEGYKWHFN ETVLTVWSAP NYCYRCGNVA AILELDEHLQ KDFIIFEAAP QETRGIPSKK PVADYFL; Positive Samples: 293T, NIH/3T3, MCF-7, HO-8910PM, Mouse testis, Mouse spleen; Cellular Location: Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center

靶标信息

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: P PPX; PP-X; pp4; PP4C; protein phosphatase 4 (formerly X), catalytic subunit; protein phosphatase 4, catalytic subunit; Protein phosphatase X; protein phosphatase X, catalytic subunit; Serine/threonine-protein phosphatase 4 catalytic subunit

基因别名: 1110002D08Rik; AU016079; PP-X; PP4; PP4C; PPH3; PPP4; PPP4C; PPX

UniProt ID: (Human) P60510, (Rat) Q5BJ92, (Mouse) P97470

Entrez Gene ID: (Human) 5531, (Rat) 171366, (Mouse) 56420

分子生物学功能: protein phosphatase