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Invitrogen
PRDX3 Polyclonal Antibody
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PRDX3 Antibody (PA3-752) in ICC/IF
Immunofluorescence analysis of Peroxiredoxin 3 was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PRDX3 Rabbit Polyclonal Antibody (Product # PA3-752) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conju... View More
产品信息
PA3-752
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified human recombinant Peroxiredoxin 3.
偶联物
形式
Liquid
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
PA3-752 detects Peroxiredoxin 3 protein in human samples. This antibody is specific for Prx 3 and shows no cross-reactivity with other Prx isoforms.
PA3-752 has successfully been used in Western blot, and immunocytochemistry procedures. By Western blot, this antibody detects an ~27 kDa protein representing Prx 3 in human PC3 cell lysate.
The PA3-752 antigen is purified human recombinant Prx 3 from HEK 293 cells.
靶标信息
Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20-30 kilodalton in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
