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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PSMB9 Antibodies

          Invitrogen

          PSMB9 Monoclonal Antibody (OTI1C4)

          查看其他13个PSMB9抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite PSMB9 Monoclonal Antibody (OTI1C4)

          • 抗体检测数据 (17)
          PSMB9 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          PSMB9 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 17

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          PSMB9 Antibody (MA5-25887) in ICC/IF

          Immunofluorescence analysis of Proteasome subunit beta type-9 was performed using 70% confluent log phase SK-O-V3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 45 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PSMB9 Monoclonal Antibody (OTI1C4) (Product # MA5-25887) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody,... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          PSMB9 Antibody in Immunocytochemistry (ICC/IF)
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PSMB9 Antibody in Western Blot (WB)
          PSMB9 Antibody in Western Blot (WB)
          PSMB9 Antibody in Western Blot (WB)

          产品信息

          MA5-25887

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:2,000
          -

          免疫组化(石蜡) (IHC (P))

          1:150
          -

          免疫细胞化学 (ICC/IF)

          1:100
          -

          流式细胞分析 (Flow)

          1:100
          -
          产品规格

          种属反应

          Human, Mouse

          宿主/亚型

          Mouse / IgG2b

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          OTI1C4

          抗原

          Human recombinant protein fragment corresponding to amino acids 21-219 of PSMB9 produced in HEK293T cell
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Affinity Chromatography

          保存液

          PBS, pH 7.3, with 1% BSA, 50% glycerol

          内含物

          0.02% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2725132

          靶标信息

          The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC in the immunoproteasome. Proteolytic processing is required to generate a mature subunit.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: large multifunctional peptidase 2; large multifunctional protease 2; LMP-2d; Low molecular mass protein 2; Macropain chain 7; MGC70470; Multicatalytic endopeptidase complex chain 7; proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2); proteasome catalytic subunit 1i; Proteasome chain 7; proteasome subunit beta 6i; proteasome subunit beta type 9; Proteasome subunit beta type-9; Proteasome subunit beta-1i; proteasome-related gene 2; proteosome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2); proteosome beta 9 subunit; Really interesting new gene 12 protein

          查看更多 收起

          基因别名: beta1i; Lmp-2; LMP2; PSMB6i; PSMB9; RING12

          查看更多 收起

          UniProt ID: (Human) P28065, (Mouse) P28076

          查看更多 收起

          Entrez Gene ID: (Human) 5698, (Mouse) 16912

          查看更多 收起

          功能
          threonine-type endopeptidase activity protein binding proteasome binding endopeptidase activity peptidase activity hydrolase activity protease
          参与通路
          MAPK cascade protein polyubiquitination liver development stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent regulation of cellular amino acid metabolic process muscle atrophy anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway response to drug proteasome-mediated ubiquitin-dependent protein catabolic process response to alkaloid regulation of mRNA stability spleen development thymus development T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway cellular response to electrical stimulus cellular response to interleukin-1 negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway cellular response to virus response to benzene regulation of cysteine-type endopeptidase activity immune system process proteolysis proteasomal ubiquitin-independent protein catabolic process antigen processing and presentation proteolysis involved in cellular protein catabolic process
          It has to be done as per old AB suggested Products section.
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          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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