Invitrogen

PSMB9 Monoclonal Antibody (OTI1C4)

PSMB9 Antibody in Immunocytochemistry (ICC/IF)

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PSMB9 Antibody (MA5-25887) in ICC/IF

Immunofluorescence analysis of Proteasome subunit beta type-9 was performed using 70% confluent log phase SK-O-V3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 45 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PSMB9 Monoclonal Antibody (OTI1C4) (Product # MA5-25887) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody,... View More

PSMB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-25887

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:150

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:100

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

OTI1C4

抗原

Human recombinant protein fragment corresponding to amino acids 21-219 of PSMB9 produced in HEK293T cell

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2725132

靶标信息

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC in the immunoproteasome. Proteolytic processing is required to generate a mature subunit.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: large multifunctional peptidase 2; large multifunctional protease 2; LMP-2d; Low molecular mass protein 2; Macropain chain 7; MGC70470; Multicatalytic endopeptidase complex chain 7; proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2); proteasome catalytic subunit 1i; Proteasome chain 7; proteasome subunit beta 6i; proteasome subunit beta type 9; Proteasome subunit beta type-9; Proteasome subunit beta-1i; proteasome-related gene 2; proteosome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2); proteosome beta 9 subunit; Really interesting new gene 12 protein

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基因别名: beta1i; Lmp-2; LMP2; PSMB6i; PSMB9; RING12

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UniProt ID: (Human) P28065, (Mouse) P28076

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Entrez Gene ID: (Human) 5698, (Mouse) 16912

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功能

threonine-type endopeptidase activity protein binding proteasome binding endopeptidase activity peptidase activity hydrolase activity protease

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