Invitrogen

PUM1 Polyclonal Antibody

Name: PUM1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-102944
Availability: InStock

查看其他14个PUM1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (5)

PUM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 5

PUM1 Antibody (PA5-102944) in IHC (P)

Immunohistochemistry analysis of PUM1 in mouse liver tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with PUM1 polyclonal antibody (Product # PA5-102944) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image PUM1 Polyclonal Antibody

产品信息

PA5-102944

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human PUM1(Accession Q14671), corresponding to amino acid residues E223-G273.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2852320

产品详细信息

Antibody detects endogenous levels of total PUM1.

靶标信息

PUM1 (pumilio homolog 1) is a sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. It is a member of the PUF family comprised of evolutionarily conserved RNA-binding proteins. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), and 5'-UGUANAUA-3' that is related to the Nanos Response Element (NRE). PUM1 mediates post-transcriptonal repression of transcripts via different mechanisms - acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Mutations to the gene can result in spinocerebellar ataxia 47.

仅用于科研。不用于诊断过程。未经明确授权不得转售。